Since ships are exposed to noise and vibration for a long time in seas isolated from land, the stress of workers is high, requiring studies on the improvement of the problem. In addition, the health of crew members on fishing vessels, unlike commercial ships, carrying out fishing activities is closely related to fishing performance. However, considering that most of IMO's guidelines apply to merchant ships, provisions should be made for vibration in accommodation areas of fishing vessels. Accordingly, this study, applying the revised ISO 20283-5:2016 standard and through experiment, investigated the vibration characteristics of the accommodation area for each operating conditions of a 1000-ton trawler fishing vessel. Within the scope of the study and the full-ahead mode, the vibration was highest in the engine control room (4.17mm/s) while the acceptable standard was satisfied sufficiently in all conditions. Application of the merchant vessel vibration standard to fishing vessel is expected to improve the working environment of fishing vessel crew members.