양강추출물(Alpinia officinarum, 70% ethanol extract)과 함유 주성분인 galangin은 프리라디칼소거작용과 지질과산화억제활성을 나타내었으며, H_2O_2 또는 KO_2유도 세포독성에 대해서도 억제적으로 작용하여 세포보호효과를 나타내었다. DNA single strand breakage와 같은 산화적 스트레스에 대해서도 보호작용을 하고 있다. 또한, 마우스 소핵시험에 의하여 adriamycin과 같은 superoxide유발물질에 의한 염색체 수준에서의 손상에 대해서도 억제효과를 나타내었다. 양강추출물은 galangin을 유효성분으로하여 산소프리라디칼들에 의한 산화적 손상에 억제적으로 작용하는 기전으로 산화적 스트레스에 대한 항노화, 암예방제로서의 응용가능성이 높은 추출물로서 향후 기능성 식품으로서의 응용가능성이 높을 것으로 판단되었다.

Background : Alpinia Officinarum Rhizome (高良薑) is recorded as a roots of Alpinia officinarum Hance (Zingiberaceae) in Korea Pharmacopoeia and there is no established confirmation test and method for quantitative determination using marker compound. Using the six marker compounds, pinocembrin, (5S)-5-hydroxy-7- (4-hydroxy-3-methoxyphenyl) -1-phenyl-3-heptanone, pinocembrin, galangin, kaemferide, galangin 3-methyl ether, A simultaneous analysis method was developed to utilize the quality control. Methods and Results : In this study, YMC-pack column (ODS C18, 250 × 4.6 ㎜, 5 ㎛) from YMC was used and the temperature was set at 30℃. A soultion of 0.1% formic acid was using water (mobile phase A) and acetonitrile (mobile B), respectively, and the mobile phase B was set to 15 - 35% for 0 - 15 minutes and 35 - 40% for 15 - 60 minutes. And 270 ㎚ was set as the detection wavelength. The order of the components is pinocembrin (1, Rt: 23.2 min), (5S)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (2, Rt: 37.8 min), pinocembrin (3, Rt: 44.9 min), galangin (4, Rt: 46.7 min), kaemferide (5, Rt: 48.6 min), galangin 3-methyl ether (6, Rt: 52.8 min) and Based on the retention time, ultraviolet spectrum and MS value, it was confirmed by comparison with standard compounds. Conclusion : In this study, we developed the simultaneous analysis method by six marker components of the Alpina officinarum Hance extract to develop a quality test method to scientifically identify Alpinia officinarum Hance.