In this study, the antioxidative activity of Jeolpyun containing S. glauca (Bunge) Bunge powder (2%, 4%, 6%, and 8%) and extract (2%, 4%, 6%, and 8%) was evaluated based on the total polyphenol contents, electron-donating ability, scavenging of superoxide anion radical, and decomposition of hydrogen peroxide. Total polyphenol contents of Jeolpyun containing 8% S. glauca (Bunge) Bunge powder were the highest with a value of 2,280±0.00 μg GAE/mL. It was observed that total polyphenol content exhibited a significant relation with concentrations at p<0.05. The electron-donating ability of Jeolpyun containing 8% S. glauca (Bunge) Bunge extract was the highest with a value of 83.93±0.69%. The electron-donating abilities revealed significant differences between concentrations (p<0.05). The superoxide radical scavenging ability of the blank was 0.01523±0.00068 and Jeolpyun containing 6% S. glauca (Bunge) Bunge powder was the highest with a value of 0.00977±0.00842. The hydrogen peroxide decomposing ability of Jeolpyun containing 0% S. glauca (Bunge) Bunge powder was －0.00377±0.0049; 8% S. glauca (Bunge) Bunge powder revealed the highest decomposing ability of －0.0476±0.0234. Hydrogen peroxide decomposing abilities revealed significant differences between concentrations (p<0.05). Jeolpyun containing S. glauca (Bunge) Bunge powder and extract demonstrated improved antioxidative activities.

In this study, S. glauca (Bunge) Bunge extract was testified total polyphenol contents, electron donating ability on DPPH, and scavenging ability of superoxide anion radical and hydrogen peroxide. Total polyphenol contents of S. glauca (Bunge) Bunge extract were 7,053.30±915.93 μg GAE/mL. In the fractions, fraction by ethanol was the highest content of 10,973.30±1,000.24 μg GAE/mL. Fractions of ethyl acetate and water were 2,386.70±166.53 μg GAE/mL, 2,413.30±41.63 μg GAE/mL respectively. It was shown that total content of polyphenol according to solvent was significant relation at p<0.05. In the experiment of the electron donating ability, 70% ethanol extract and methylene chloride fraction were －246.15±24.17%, －254.01± 16.54% respectively. In case of ethyl acetate fraction, it was the highest electron donating ability to DPPH radical, 39.06±0.34%, and then water fraction of 36.71±5.55%, ethanol fraction of 29.77±2.57%, gradually. Electron donating abilities revealed significant difference (p<0.05) between the solvents. The superoxide radical scavenging ability of standard material was 0.029±0.0011; 70% ethanol extract of 0.022±0.00052, methylene chloride fraction of 0.027±0.00031, ethyl acetate fraction of 0.024±0.0011, ethanol fraction of 0.021±0.00024 and water fraction of 0.024±0.00019. Hydrogen peroxide scavenging ability of 70% ethanol extract was －0.0029±0.00040 and the others were as follow; methylene chloride fraction (－0.0042±0.00058), ethyl acetate fraction (－0.003± 0.0041), ethanol fraction (－0.0029±0.0015) and water fraction (－0.0028±0.00090).

Extraction of Sedum sarmentosum Bunge by super critical carbon dioxide was operated under 40-50℃and 200-250 atm, thus, yield of extraction was very low as 4 wt%. Rats were administrated with the extract orally once a day for succesive 6 days, followed by treatment with carbon tetrachloride (CCl4) on the sixth day. The activities of aminotransferase, alkaline phosphatase, γ-glutamyl transpeptidase, lactate dehydrogenase and contents of triglyceride, total cholesterol in the extract-pretreated rats were decreased compared to the CCl4controled rats, whereas content of HDL-cholesterol was increased. Especially content of hepatic malondialdehyde (MDA) and atherogenic index (AI) were decreased and HTR was increased in the extract-pretreated rats, and reduced peroxidative liver damage in the CCl4-induced hepatotoxicity rats. In addition, activities of hepatic superoxide dismutase, catalase, glutathione peroxidase in the extract-pretreated rats were significantly decreased compared to the CCl4 controled rats, but the content of glutathione was significantly increased. These results suggest that extract of Sedum sarmentosum Bunge has hepatoprotective effect in the CCl4-intoxicated rats.

To evaluate the protective effect of Ajuga multiflora BUNGE (AMB) extract on the toxicity of lead acetate (LA), environmental pollutant, cell viability was measured by XTT assay using cultured NIH3T3 fibroblasts. And also, the effect of antioxidant, butylated hydroxytoluene (BHT) on LA-induced cytotoxicity was analysed. For the protective effect of AMB extract on LA-induced cytotoxicity, NIH3T3 fibroblasts were pretreated with 80 or 90 μg/mL of AMB extract for 2 hours before the treatment of LA. And also, the antioxidative effects of AMB extract against LA-induced cytotoxicity were assessed by DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP). In this study, LA significantly decreased cell viability dose-dependently compared with control, and then XTT50 value was determined at 46.1 μM of LA. In the effect of BHT against LA-induced cytotoxicity, it effectively prevented toxic effect of LA by the significant increase of cell viability. In the protective effect of AMB extract on LA-induced cytotoxicity, it significantly increased cell viability which was decreased by LA-induced cytotoxicity, and also it showed the antioxidative effects such as DPPH-radical scavenging activity, SOD-like activity and inhibitory activity of LP. From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and AMB extract effectively prevented the cytotoxicity induced by LA via an antioxidative effect. Conclusively, the natural substance such as AMB extract may be alternative resources for the prevention or treatment of diseases related with oxidative stress.