Somatic cell nuclear transfer (SCNT) technique is a key point of producing transgenic animal disease models. During in vitro production of SCNT embryo, the quality of matured oocytes are one of the important factors that regulate embryo developmental capacity. In preliminary test, we confirmed the effect of fibroblast growth factor 10 (FGF10) on porcine oocyte maturation. In this study, we investigated the developmental potential of SCNT embryos treated with the 10 ng/ml FGF10 (10 F) during in vitro maturation of recipient oocytes. The polar body emission rate was significantly higher in the 10 F treated group than control group. After SCNT, although the rate of fusion was no significant difference, the rate of cleavage and blastocyst formation was significantly increased in the 10 F treated group (p<0.05). In 10 F treated group, the total cell number was increased and the percentage of apoptotic cell was decreased in the blastocyst stage at day 7 (p<0.1). The transcription level of apoptosis relative gene, Casp3 was significantly decreased, while anti-apoptosis gene BCL2l1 was increased in the 10 F treated group compared to control group. The 10 F treated group was highly expressed the reprogramming related genes, Sox2 and POU5f1. Also, the first cleaving time was more faster and the percentage of cell block was significantly lower in 10 F treated group than in control group. In this study, we confirmed that 10 ng/ml FGF10 has effect on enhance the oocyte maturation and developmental capacity. These results demonstrate that FGF10 treatment can be used for in vitro development of porcine SCNT embryos and subsequent production of transgenic animal model.

현행 내진설계에서 반응수정계수는 탄성 밑면전단력을 저감하여 설계하중 수준을 정의하기 위한 주요 계수로 사용되고 있다. 이제까지 반응수정계수는 공학자들의 경험적인 합의에 의하여 정성적으로 설계기준에 반영하고 있다. 구조시스템에서 반응수정계수와 접합부의 가용 회전능력은 매우 밀접한 관계가 있으며, 본 논문에서는 이러한 접합부의 회전능력과 비선형 푸쉬오버 해석에 기초하여 반응수정계수를 평가하는 방법을 제시하였다. 이를 검증하기 위하여 IBC 2000에 따라 설계된 R3S 골조를 대상으로 제안 방법을 적용하였다. 또한, 다양한 지진파에 대한 비선형 시간이력 해석을 병행하여 가용 회전능력에 의거하여 산정된 반응수정계수의 타당성을 평가한 결과, 본 제안방법에 따라 정의된 반응수정계수가 충분히 보수적임을 확인하였다.

This experimental study evaluates of seismic performance on strain-hardening cement-based composite (SHCC). The objective of this study is to evaluate of seismic capacity reduction factor on two sides confined SHCC infill walls. The experimental results as cseismic capacity reduction factor are not significantly different.

This study investigated the correlation of physique factor( standing height, body weight, chest girth, body surface ) and vital capacity upon records of swimming discipline at sea water. The subjects are 194 sophomore students of Korea Maritime University who participated in the swimming discipline. The swimming records were divided into three groups - higher, middle and lower group. The results from this investigation are as follow ; 1. The standing height was above the average on the A, B+ record, body weight was above on the A+, A record, and vital capacity was above on the A+, A, B+, C record respectively. 2. The standing height, body weight, chest girth, body surface, vital capacity was significantly correlated between record P < 0.05 and P < 0.01. Therefore the higher record group has better physique factor and vital capacity than the middle or lower record group. 3. The standing higher of the higher record group was significantly correlated with body weight(0.514), body surface(0.768) and vital capacity(0.427), and body weight was significantly correlated with chest girth(0.525), body surface(0.940) and vital capacity. This standing heiht of the middle record group was sigificantly correlated with body weight(0.509) and body surface(0.779), and body weight was significantly orrelated with chest grith(0.618) and body surface(0.927). The standing height of the lower record group was significantly correlated with body weight(0.595), chest grith(0.363), body surface(0.802) and vital capacity(0.250), and body weight was significantly correlated with chest grith(0.678) and body surface(0.952).