The Yangtze River Delta region of China takes the lead in the protection of traditional crafts across the country. Studying the current situation of its policy system is of great significance for the protection of intangible cultural heritage not only in the Yangtze River Delta but also throughout China. Focusing on the traditional craft protection policies in the Yangtze River Delta, this study selected 64 relevant policy texts from 2000 to the present. Using NVivo 11 software, it conducted coding extraction and qualitative analysis on 188 free nodes and 29 tree nodes under 10 core categories covered in the policies, including the concept and directory system, legal subjects' rights and interests, the concept of hierarchical protection, talent team building, fund investment, resource utilization, multi-field collaboration, research deepening, digital technology support, and work coordination and supervision mechanisms.The research results show that the traditional craft protection policy system in the Yangtze River Delta region has presented diversified policy orientations, a relatively complete four-level structure, differentiated cross-border integration characteristics, and an initially formed regional collaboration mechanism. However, there are still certain limitations in aspects such as the precision of rights and interests protection, the explicit integration of digital technology, the construction of a sustainable path for "productive protection", the improvement of the closed-loop evaluation and supervision system, and the in-depth expansion of regional collaboration.

XIST has been known to long-non coding RNA which regulate X-chromosome inactivation in female mammal and the gene has been suggested to having important role in early embryo development and embryonic stem cell. However, its coding region has been unclear in pig. To determine the coding region of XIST in pig, we have examined candidate site of XIST coding region in pig by BLAST, PCR, and sequencing. By comparing pig whole genome sequence (Sus scrofa 10.2) with human, murine, and bovine XIST transcript sequence using BLAST, we selected candidate coding region of XIST in pig. The result showed XIST is coded on the minus strand of NW_003612825 contig and its length was nearly 32kb which was similar to the length of human and bovine XIST gene. With the candidate model, we performed RT-PCR to confirm the coding region of XIST with 24 primer pairs and they were expressed only female porcine embryonic fibroblast (PEF) but not in male PEF. By designing candidate intron spaning primer we could confirmed candidate intron is present between first and last exon (distance, 9.2kb vs product size, 2kb). The seqeucne of amplicon was analyzed and we could confirmed there were 5 small exons (less than 400 bp) like XIST coding region of other species which have 4 to 5 small exon between first and last exon. To confirm coding strand in pig, we conducted strand specific reverse transcription. We confirmed candidate XIST was coded on the negative strand of contig on X-chromosome as the result of homology analysis by BLAST. With the candidate pig XIST sequence, we aligned the sequence with XIST sequences of 3 species, human, mouse, and bull by clustalW. These result showed candidate sequence of pig XIST is most similar to that of bovine and the homology between pig and human was higher than result between mouse and human. These results could support for X chromosome inactivation analysis and the function of XIST in pig preimplantation embryos. * This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2012006276)

과학기술이 빠르게 변화하고 있는 시대에 선진국에서는 코딩 교육의 중요성을 인식하고 미래형인재를 양성하기 위해 많은 노력을 기우리고 있다. 미국의 경우 버락 오바마 대통령의 ‘모든 이에게 컴퓨터 과학을(Computer Science for All)’이라는 슬로건을 중심으로 코딩 교육에 적극투자를 하고 있고, 핀란드와 영국은 4-5세의 유아들이 코딩을 배우며 미래를 준비하고 있다.2) 하지만 IT강국인 우리나라의 경우는 최근에서야 코딩 교육의 중요성을 인식하고 지난 2015년에서야 개정된 교과과정에 따라 코딩교육을 의무화하고 있다. 이에 유아를 대상으로 한 코딩교육에 대한 관심도가 높아짐에 따라 아이들에게 새로운 언어와 사고법으로 놀면서 코딩 개념을 가르치는 다양한 유아 교구들이 등장하고 있다. 그 중에서 스마트기능이 탑재된 토이형태의 교구들이 학부모들에게 큰 관심을 받고 있다. 하지만 타 교구에 비해 스마트 토이는 고가의 교구인데다 흥미위주로만 만들어진 교구들이 많아 문제해결 능력과 사고력을 증대시키는 요소와 유아들의 어포던스가 잘 반영된 교구에 대한 연구가 미흡한 상황이다. 따라서 본 논문은 코딩교육을 위한 스마트토이를 중심으로 유형을 분류하고 국내 인터넷에서 판매순위가 높은 스마트 토이를 대상으로 어포던스 요소별로 사례 분석하고 코딩 교육이 나아가야할 방향을 제시하였다. 그 결과, 감각적 어포던스는 높은 수치를 보였으며 타 과목에 비해 자신이 직접 만든 장남감이라는 인식이 강해 생명력을 느끼며 흥미를 갖는 것으로 판단된다. 반면, 물리적 어포던스는 낮은 수치를 보였는데 이는 직접 조립하고 연결하는 방식에 대한 흥미는 상대적으로 타 장남감에 비해 흥미도가 떨어지는 것을 유추할 수 있다.