Tomato fruits(Lycoperisicon esculentum) synthesize the glycoalkaloids dehydrotomatine and α-tomatine, possibly as defense against bacteria, fungi and insects. We developed a new effective method to prepare and purify dehydrotomatine and α-tomatine that exists in tomato fruits using alumina column chromatography and high performance liquid chromatography (HPLC). The tomato glycoalkaloids(TGA) in tomato was extracted with 2% acetic acid, and then precipitated with ammonium hydroxide(pH=10.5). The dry precipitate substance was applied on alumina column, and then fractionated with water saturated n-butylalcohol. The TGA(Fr. No. 26~36) were collected and dried under reduced pressure. The TGA was performed on a reverse phase HPLC(Inertsil ODS-2, 5 ㎛), eluted with acetonitrile/20mM KH2PO4(24:76, v/v) at 208 ㎚. Two peaks were detected on HPLC, and individual peak was collected by repeating HPLC. Furthermore, to confirm the identity dehydrotomatine and α-tomatine, each peak isolated was hydrolyzed with 1N HCl into sugar and aglycone tomatidine. The sugars were converted to trimethylsilyl ester derivatives. The nature and molar ratios of sugars were identified by gas-liquid chromatography(GLC) and the aglycone by high-performance liquid chromatography(HPLC). The first peak (Rt=17.5 min) eluted from HPLC was identified as dehydrotomatine, and second peak(Rt=21.0 min) was as α-tomatine. This technique has been used effectively to prepare and isolate dehydrotomatine and α-tomatine from tomato fruits.

난포액내 함유되어 있는 단백질성분 중에서 sucrose 층으로부터 정자의 swim-up 이동을 자 극하는 성분을 분리하기 위하여 paper chromatography (PC) 및 reverse phase column (RPC) 과 superose column (SC)를 이용한 액체 chromatography의 분리효과를 조사하였던 바 결과는 다음과 같다. 1. Chromatography용 paper로 분리한 각 band 의 성분은 첨가농도가 증가할수록

Background : Resveratrol is the stillbenoid material made by the stress factors from the plants like grape, blueberry and Polygonum cuspidatum and it has useful pharmacological activity such as anti-cancer and longevity effect. Although grapevine contains less resveratrol compared to Polygonum cuspidatum, it is expected that a large amount of resveratrol can be obtained using by-products from the processing because grapes are one of the crops grown and processed considerably in south Korea. Methods and Results : As the result of the analysis of resveratrol content in grape extract using high-performance liquid chromatography (HPLC), it was showed that the amount of the resveratrol was 46.8~47.8 mg(about 4.7~4.8%) per 1 g of grape extract. The method used for purifying resveratrol from grape extract was a column chromatography and adsorption resin was used in packing the column. The content of resveratrol in the eluate obtained by column chromatography was analyzed by HPLC. Then the solid content obtained by freeze-drying was analyzed to get the purity and the collect rate of the resveratrol by measuring the weight. The result showed that the purity of the resveratrol in eluate obtained by column chromatography was measured to 15.2~18.1% and collect rate was up to 95%. Conclusion : From the above results, we have isolated resveratrol which is more pure than the conventional from grape extract and assumed that it is helpful to produce functional material at a low cost by using this isolation method.