In the present study, we investigated the effects of artificial removal of corolla and calyx lobes from open flowers on fruit development and fruit quality of strawberry (Fragaria × ananassa Duch.). Removing the corolla had no significant effect on fruit weight, hardness, length, and diameter, Hunter L, a, b values, sugar content and acidity. However, removing the calyx lobes significantly inhibited longitudinal and transverse growth of the fruits, resulting in diminished fruit size. In addition, merchantable fruit quality significantly decreased upon removing the calyx lobes, and this tendency was increased as more calyx lobes were removed. Nonetheless, removal of the calyx lobes had no effects on fruit color, sugar content, or acidity, similar to the effect of corolla removal.