본 연구는 세계적으로 점점 애완 식품 시장이 발달하고 있음에 따라 천연물을 소재로 하여 치석, 치태를 감소시킬 수 있는 기능성 수제 간식을 개발하고 그것을 통하여 구강의 건강을 개선하고자 진행하였다. 연구에 사용된 개들은 주변 학우들에게 연구에 대하여 설명을 해주고 동의한 학우들의 개들에 통하여 진행하였다. 간식 급여 기간은 2016년 5월 11일 ~ 25일까지 2주간 급여를 진행하였고, 체중을 기준으로 급여하였다. 지표를 이용하여 치석, 치태의 축적도를 관능검사로 평가하였다. 상이한 대상을 통한 연구로 인하여 정확하지 못한 실험 결과를 초래 하였다. 이를 확인하기 위한 적절한 동물들의 사용과 재료에 대한 연구와 실험방법, 실험기간을 제대로 확립한다면 치석, 치태 감소에 좋은 새로운 소재라 생각한다.

TM7 is an uncultivated organism which is present in ex- tremely diverse environments. Members of the Dialister genus are difficult to culture as a result of which many of these strains remain uncultivated. It has been suggested that TM7 and Dialister bacteria may belong to a group of suspected periodontal pathogens. In our current study, the presence of the sebacteria in Korean dental plaque samples was asses- sed using PCR detection methods with specific primers for 16S ribosomal RNA genes. The experimental group inclu- ded 84 volunteers (35 males and 49 females). Plaque sam- ples were collected from 4 non-adjacent proximal sites of the molar areas of the mandible in each subject and pooled. TM7 was detectable in 56% and the Dialister genus in 27.5% of the volunteers. Both TM7 and Dialister were present in 20.3% of volunteers. We found that 36.9% of the volunte e rs we re negative for both bacteria. Further s tudi e s to evaluate the prevalence of these putative pathogenic bac- te ria in the Korean population are warranted.

It has been reported that the antimicrobial susceptibility patterns of viridans streptococci vary according to geographical region. Although several studies on the antibiotic resistance of viridans streptococci in foreign countries have been reported, little is known about the distribution of resistance among viridans streptococci in Korea. In this study, 88 isolates of viridans streptococci from Korean students' dental plaque were identified as 12 different species. The susceptibility of these isolates to 8 antibiotics was investigated. The in vitro antibiotic activity of penicillin G, ampicillin, vancomycin, streptomycin, gentamicin, erythromycin, amoxicillin, and tetracycline was measured by the broth microdilution method. The range of the minimum inhibitory concentrations (MIC), MIC50, MIC90, and the percentage of the susceptible isolates were determined. Streptococcus mutans and Streptococcus salivarius were susceptible to the 8 antibiotics. Isolates with resistance to vancomycin, streptomycin, and amoxicillin were not found. The overall resistance rates of the 88 isolates to penicillin G, ampicillin, gentamicin, erythromycin, and tetracycline were 12.5%, 62.5%, 62.5%, 26.1%, and 26.1%, respectively.