This study was conducted to examine the effect of a diet supplemented with dried Bacillus subtilis culture on broilers' productivity and blood characteristics. Broiler feeding experiments were conducted twice. The diet fed to the control group was supplemented with 0.2% Palm MateⓇ commercially available probiotics (B. subtilis), and the diet fed to the experimental group was supplemented with ThekerⓇ 0.05% dried B. subtilis culture (DBC) in experiment 1, and 0.1% DBC in experiment 2. Treatment was administered for four weeks in both groups. A higher average daily gain was observed in the group treated with 0.05% DBC in experiment 1 compared with the control group, which was significantly higher in the group that received 0.1% DBC in experiment 2 compared with the control group. A higher production index was observed in the groups that received treatment than in the control group and was higher in the 0.1% DBC group than in the 0.05% DBC group. Significantly lower serum triglyceride (TG) was observed with increased DBC content. Although the findings showed no statistical significance, lower total cholesterol (T-C) was observed in the treated group than in the control group. HDL-cholesterol (HDL-C) content showed a significant increase in the DBC-treated groups. A significantly opposite outcome was observed for LDL-cholesterol (LDL-C) content. These findings demonstrated that the atherogenic index (AI) and cardiac risk factor (CRF) decreased in the DBC-treated groups. Significantly lower serum levels of glutamic-oxaloacetic transaminase (GOT: aspartate aminotransferase, AST) and glutamate pyruvic transaminase (GPT: alanine transaminase, ALT) were detected with an increase in DBC content in the treated groups compared with the control groups. To summarize the findings described above, adding ThekerⓇ dried B. subtilis culture to broiler feeds positively improved productivity through weight gain of broilers and the production of healthier functional broilers through the improvement of blood lipids and liver function. It is expected that the findings of this study will be helpful in the effort to increase the profitability of broiler chicken farming and promote human health.

두 종류의 Cronobacter 선택배지(DFI agar, R&F agar) 의 분유 및 건조호박 내 Cronobacter의 선택 분리능을 realtime PCR법과 함께 비교하였다. 분유에서의 Cronobacter 검출률은 세 가지 방법에서 유의적인 차이를 나타내지 않았으나(p < 0.05), 건조호박의 경우 R&F배지와 real-time PCR법이 DFI에서보다 유의적으로 높은 검출률을 보였다 (p < 0.05). 배지 간 선택성에 있어서도, R&F 선택배지는 건조호박에서 DFI에 비해 유의적으로 높은 선택성을 나타냈다(p < 0.05). Real-time PCR 및 R&F배지의 사용은 분유뿐만 아니라, 건조 호박 등의 높은 경쟁세균총을 갖는 영유아식의 원료로 사용될 수 있는 식품군에서도 Cronobacter를 효과적으로 검출할 수 있는 방법으로 사료된다.