Background: Porcine embryonic development is widely utilized in the medical industry. However, the blastocyst development rate in vitro is lower compared to in vivo . To address this issue, various supplements are employed. Extracellular vesicles (EVs) play the role of communicators that carry many bioactive cargoes. Additionally, the contents of EVs can vary on the estrous cycle. Methods: We compared the effects of adding EVs derived from porcine uterine fluid (UF), categorized as non-EV (G1), EVs in estrus (G2) and EVs in diestrus (G3). After in vitro culture (IVC) was performed in three different groups, cleavage rate and blastocyst development rate were examined. In addition, glutathione (GSH) and reactive oxygen species (ROS) levels were measured 2 days after activation to assess oxidative stress. Results: Using NTA and cryo-TEM, we confirmed the presence of EVs with sizes ranging from 30 nm to 200 nm, that the particles were suitable for analysis for analysis. In IVC data, the highest cleavage rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Similarly, the highest blastocyst development rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Conclusions: These results indicate that estrus derived EVs contain biofactors beneficial for early blastocyst development, including GSH which protects the blastocyst from oxidative stress. Additionally, although diestrus-derived EVs are expected to have some effect on blastocyst development, it appeared to be less effective than estrus-derived EVs.

Purpose - The purpose of this study is to classify that the quality factors for comparing the Visegrard Group with the Nordic Council have historical similarities against Germany and the Soviet Union. However, this is because in the integrated European market, the competitiveness possessed by the two groups of countries is invested in the priority order to grow. Research design, data, and methodology – The study was conducted on the research design, and the reason for trying to compare the competence of the automobile industry in the assessment of industrial capability is that the Visegrard Group focuses on automotive production and the Nordic Association focuses on the commercialization of the automobile(market). In this study, searching and quantifying indirect evidence was made through standards are more complementary in Europe since each country acts like the role of the European automotive industry for example, which is different from the realistic evaluation criteria, are more important than those of the United States(first in the world) or Germany(first in Europe). Results - The results of this study are as follows: In the global EV market U.S.(export: $ 2.62 billion /share: 36.7%), Germany($ 1.29 billion /18.1%), France($ 390 million /5.4%), United Kingdom($ 380 million /5.4%), and South Korea($ 320 million/ 4.4%). South Korea's share of the EV market is 4.4%, while TSI reaches at +0.9 which measures the comparative advantage of a specific commodity in the world trade market. There is great potential for evaluated as products processing in export competition. But, commercialization, standardization, and overall market expansion did not have a positive impact on global satisfaction. Conclusions - EVs put importance on various utilities. So this suggests that Korea's exports to the EU, including the Visegrard Group, should be more focused on marketability when illuminating with a sharing industrial system under the European Union. It is necessary to specialize in manufacturing and commercialization by country(region) to prepare sharing economy and blockchain in order to create a smart-sharing city linked on artificial intelligence, as the commercialization of electric vehicles, which will have a larger growth rate than that of manufacturing in the fourth revolutionary era.