The onion thrips, Thrips tabaci (Thysanoptera: Thripidae), is a worldwide pest that causes serious damage to Allium crop species and acts as a vector for iris yellow spot virus (IYSV). In a previous study, we established an emamectin benzoate (EB) resistant strain (EB-R) with a 490-fold higher resistance ratio than the susceptible strain (SUS). The EB-R exhibited significantly increased transcript levels of glycine receptor alpha, glutamate-gated chloride channel (GluCl) b, and cytochrome P450 (CYP450) 6EB2 compared to SUS. To identify EB resistance-related genes that are differentially expressed genes between SUS and EB-R, we established an isogenic backcrossing strain and conducted transcriptome analysis after the 4th cycle of isogenic backcrossing. Among the 85 up-regulated genes in the transcriptome data, six cuticular protein genes showed up-regulation. Additionally, CYP450 4g15, which catalyzes the synthesis of cuticular hydrocarbons, exhibited a 6 log2-fold higher expression level in EB-R compared to SUS. Therefore, the elevated expression of genes associated with cuticle protein modification may be significantly is involved in the development of EB resistance.

솔껍질깍지벌레는 소나무재선충에 의한 소나무 재선충병과 더불어 남부해안지역의 곰솔림에 심각한 피해를 주는 해충으로 일부 지역에서는 두 병해충이 동시에 발생하고 있다. 산림 내 병해충 방제는 대면적이고, 방제의 제한점이 많아 두 종 이상의 병해충을 동시방제 할 필요성이 높은 편이다. 본 연구에서는 동일 기주에서 나무주사를 통한 솔껍질깍지벌레 방제효과가 우수한 약제를 선발하고, 소나무재선충병의 동시 방제를 위하여 2종의 유효성분을 혼합 처리하여 그 효과를 검정 하였다. Thiamethoxam 유제 (2.15%)와 Imidacloprid 분산성 액제 (20%)를 단독 처리한 결과 86.5%, 89.7%로 솔껍질깍지벌레에 대한 살충효과가 뛰어났으며 효과가 우수한 두 가지 살충제와 소나무재선충병 수간주사 약재인 Emamectin benzoate 유제 (2.15%)를 한 기주에 각각 주사한 결과 Thiamethoxam 유제 (2.15%) 혼합 처리구에서는 97.1%의 방제효과를 보였으나 Imidacloprid 분산성 액제 (20%) 혼합 처리구는 9.4%로 방제효과가 감소하였고, 두 약제를 섞어 한번에 주사한 경우에도 6.2%의 낮은 살충효과를 나타내었다. 따라서 솔껍질깍지벌레와 소나무재선충병 동시 방제를 위하여 Emamectin benzoate와 Imidacloprid를 혼합 처리하는 것은 부적합 한 것으로 나타났다.

This study was performed to investigate the change of probing and feeding behavior of Q type of B. tabaci according to the decrease of residual effect of two insecticide, emamectin and pyridaben, using EPG technique. Examined the residual activity during 20 days, pyridaben is showed longer than emamectin benzoate. When treated two insecticides onto tomato leaves with the recommended concentration through 20 days, EPG waveforms of Q type of B. tabaci was recorded during three hours compare with the characteristic patterns of feeding behavior between two insecticides such as duration of first probing time, total duration of non-probing phase, total duration of probing phase and total duration of phloem phase. Recorded result from the change in total duration of probing activity to react the two insecticides, pyridaben was showed higher the time of probing activity, however, total duration of phloem phase was appeared low activity. Total duration of phloem phase with passage of days did not show until seven days, however, and gradually increased in emamectin benzoate after 10 days. In conclusion, residual effect between two insecticides was showed more rapidly decreased in emamectin benzoate, however, feeding behavior of Q type of B. tabaci was increased.