Metabolomics aims at the comprehensive, qualitative and quantitative analysis of wide arrays of endogenous metabolites in biological samples. It has shown particular promise in the area of toxicology and drug development, functional genomics, system biology and clinical diagnosis. In this study, analytical technique of MS instrument with high resolution mass measurement, such as time-of-flight (TOF) was validated for the purpose of investigation of amino acids, sugars and fatty acids. Rat urine and serum samples were extracted by selected each solvent (50% acetonitrile, 100% acetonitrile, acetone, methanol, water, ether) extraction method. We determined the optimized liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) system and selected appropriated columns, mobile phases, fragment energy and collision energy, which could search 17 metabolites. The spectral data collected from LC/TOFMS were tested by ANOVA. Obtained with the use of LC/TOFMS technique, our results indicated that (1) MS and MS/MS parameters were optimized and most abundant product ion of each metabolite were selected to be monitorized; (2) with design of experiment analysis, methanol yielded the optimal extraction efficiency. Therefore, the results of this study are expected to be useful in the endogenous metabolite fields according to validated SOP for endogenous amino acids, sugars and fatty acids.

The paper is to compare two villages in Songak-myeon, Asan, Cnungnam Province in Korea. It tries to show how the joint village project, which began with the support of the same external organization, produces different results depending on the internal conditions and circumstances of the village. From the end of the 1990s, Pyeongchon-ri and Sugok-ri started developing villages under the influence of the environmentally friendly farming movement. And subsequent supports were provided by local organizations for the development of the village. However, the village project in Sugok-ri, which focused on economic performance, later turned out to be a failure, and the residents changed to pursue activities for their own economic interests individually. On the other hand, In Pyeongchon-ri, the cooperation among villagers was strengthened in implementing support projects by similar external agencies. In addition, community ties and identity have been strengthened as problems have been solved through cooperation among residents in the process of promoting the village project. Nevertheless, projects undertaken in Pyeongchon-ri for the purpose of increasing economic performance was all failed. Through these findings, the paper asserts that the neo-endogenous development approach utilizing external support on the basis of internal cooperative network among villagers must be a very useful method for rural development in contemporary societies.

This study postulates a partial mediation model(study model I) and a whole mediation model(study model II) to examine the effect on endogenous rural development of local innovation capability strengthening in the local industry promotion project, the establishment of promotion system, and the revitalization of local economy. To accomplish study purposes, 169 response samples from 85 project groups which drive the local industry promotion project were verified using SPSS 12.0 and AMOS 5.0. The results showed that in the partial mediation model, the local innovation capability strengthening had a significantly positive effect on the establishment of promotion system, the establishment of promotion system had a significant effect on the revitalization of local economy, and the revitalization of local economy had a significant effect on the endogenous rural development, while the establishment of promotion system and local innovation capability strengthening didn't have effect on the endogenous rural development. In the whole mediation model, the local innovation capability strengthening had a significant effect on the revitalization of local economy, the establishment of promotion system on the revitalization of local economy, and the revitalization of local economy on the endogenous rural development. According to the verifications of study model I and II, the endogenous rural development is achieved after the revitalization of local economy through the establishment of promotion system and the local innovation capability strengthening. From these results, this study presents suggestions, limits of study and directions in the future study.

To find out the relationship between pod development and cytokinin contents during reproductive stage of peanut, the cytokinin contents, trans-zeatin ribo-side (t-ZR) and dihydrozeatin riboside (diZR), were investigated at 0, 7, 14, 21 and 28 days after flowering (DAF). The amounts of t-ZR and diZR in cotyledon and first branch among primary branches were 3, 448 pmol/g (FW) and 4, 824 p/g (FW), respectively, which were higher than those of other branches. The t-ZR and diZR contents of lower parts on the branch from cotyledon node at 7 DAF were 579 pmol/g (FW) and 2, 028 pmol/g (FW), respectively, which were higher than those of upper parts. The cytokinin contents of reproductive organs as flowering progressed were increased at 0 and 14 DAF on branch and position of node. The cytokinin contents of upper part with pruning the lower part on the branch from cotyledon node were high 112-337% at 7 DAF and 14 DAF compared with those of the control. In case of remove the upper part of the first internode on main axis, t-ZR contents was 4.7 times higher than diZR contents at 7 DAF. The pod setting rate of flower and position on the branch from cotyledon node was closely related to the cytokinin contents during floral reproductive stage.