The extracts from Agastache rugosa O. Kuntze, their chloroform and hexane fractions, and estragole identified from hexane fraction were tested to investigate the effects on the growth and metabolic activities of several true fungi. The fungi used were: Aspergillus oryzae KFCC 890, Aspergillus niger KCCM 11240, Saccharomyces cerevisiae IAM 459?, Saccharomyces ellipsoideus PNU 2215. The growth of S. cerevisiae by treatment of water extract (1%), hexane fraction (0.05%), and estragole (0.05%) were inhibited 93%, 50%, and 33% respectively, and S. ellipsoideus was also inhibited markedly with delaying the lag phase maximum 12 hrs. The growth of A. oryzae was inhibited by treatment of extracts and fractions. The ethanol production by S. cerevisiae was increased more than two times in the highest value around 42 hrs incubation by water extract, but chloroform fraction inhibited its production. The glucoamylase activities by A. niger were strongly inhibited by hexane and chloroform fractions (0.05%). The invertase activity by S. cerevisiae using estragole (0.05%) reached to 57.5% of control group. S. cerevisiae treated with the estragole was damaged the cell wall and cell membrane, leaked the protoplasm, and observed broken pieces of cell.

Water extract, and methanol extract, its chloroform and hexane fractions, and estragole from Agastache rugosa O. Kuntze were tested to find the inhibiting effect on the growth of several microorganisms. The organisms used were: Escherichia coli ATCC 1129, Staphylococcus aureus 1AM 1011, Vibrio parahaemolyticus WP, Bacillus aubtilis ATCC 6633, Aspergillus oryzae KFCC 890, Aspergidlus niger KCCM 11240. Water and methanol extracts at the concentration of 0.5%, and chloroform and hexane fractions at the concentration of 0.05% inhibited the growth of microorganisms from 1/5 to 2/3 of the control group. Eatregole identified from the hexane fraction as a major component, its authentic compound completely inhibited the growth of Vibrio parahaemolyticus completely at the concentration of 0.03%, and the other bacteria were at 0.05% .