Abnormal HLA-G expression occurs in various diseases such as melanoma, renal cell carcinoma, asthma, and classic Hodgkin’s lymphoma. The purpose of this study was to determine whether HLA-G gene is linked with oral squamous cell carcinoma (OSCC). To investigate the possible link with susceptibility to OSCC, 54 OSCC patients and 120 healthy controls were enrolled in this study. HLA-G 14bp insertion/deletion polymorphism is in 3'-untranslated region of HLA-G gene. HLA-G 14bp insertion/deletion polymorphism was analyzed using the polymerase chain reaction (PCR) method. For the analysis of genetic data, SPSS18.0 program was used. Logistic regression models were performed for odds ratio (OR), 95 percent confidence interval (CI), and p value. There was a significant difference in distribution allele between OSCC patients and control subjects (OR=0.018, 95% CI=0.002- 0.131, p<0.001). Our results suggest that HLA-G 14bp insertion/deletion polymorphism may be linked with susceptibility to OSCC in the Korean population.

Many transgenic domestic animals have been developed to produce therapeutic proteins in the mammary gland. However, purification of therapeutic proteins from transgenic milk are very important for productivity of recombinant protein. Development of a knock-in vector system is needed to improve production of therapeutic proteins. In this study, we are develop Knock-in vector to express human Erythropoietin protein (hEPO) using Gluthathione S-transferase (GST) fusion system on mouse β-casein exon 3 locus. The knock-in vector consisted of the 5 homologous arm (1.02 kb), GST, PreScission protease site, hEPO cDNA, BGH polyA signal, CMV-EGFP, and 3homologous arm(1.81 kb). The analysis of nucleotide and amino acid sequence revealed that GST-hEPO mRNA is probably translated with the mouse β-casein sequence and the β-casein-GST-hEPO fusion protein is probably secreted by ER-Golgi pathway. After that, the hEPO protein can be cleaved to remove the GST from the fusion protein by PreScission protease during purification of recombinant protein. This knock-in vector may help to create transgenic mouse expressing human Erythropoietin protein via the endogenous expression system of the mouse β-casein gene in the mammary gland.

Genomes of clusters of related eukaryotes are now being sequenced at an increasing rate. In this paper, we developed an accurate, low-cost method for annotation of gene prediction and exon-intron structure. The gene prediction was adapted for delta 1-pyrroline-5-carboxylate-synthetase (p5cs) gene from China wild-type of the halophytic Leymus chinensis (Trin.), naturally adapted to highly-alkali soils. Due to complex adaptive mechanisms in halophytes, more attentions are being paid on the regulatory elements of stress adaptation in halophytes. P5CS encodes delta 1-pyrroline-5-carboxylate-synthetase, a key regulatory enzyme involved in the biosynthesis of proline, that has direct correlation with proline accumulation in vivo and positive relationship with stress tolerance. Using analysis of reverse transcription-polymerase chain reaction (RT-PCR) and PCR, and direct sequencing, 1076 base pairs (bp) of cDNA in length and 2396 bp of genomic DNA in length were obtained from direct sequencing results. Through gene prediction and exon-intron structure verification, the full-length of cDNA sequence was divided into eight parts, with seven parts of intron insertion. The average lengths of determinated coding regions and non-coding regions were 154.17 bp and 188.57 bp, respectively. Nearly all splice sites displayed GT as the donor sites at the 5' end of intron region, and 71.43% displayed AG as the acceptor sites at the 3' end of intron region. We conclude that this method is a cost-effective way for obtaining an experimentally verified genome annotation.