Cicadas that produce loud calling songs for mate attraction can be a nuisance to city dwellers in Korea. The exuviae of final instars can be used for estimation of population density. To provide a key for species identification, we investigated morphological characteristics of final instar exuviae for four cicada species that are abundant in urban areas of central Korea: Cryptotympana atrata, Meimuna opalifera, Oncotympana fuscata, and Graptopsaltria nigrofuscata. Eight morphological characters were used for statistical analyses. The results of a general linear model showed that species and sex were significant for morphological characters and that mean values of most of the morphological characters measured were significantly different among four cicada species. C. atrata and M. opalifera were separated from the other species in most characters measured. Although O. fuscata and G. nigrofuscata were not distinguishable based on size-related characters, these two species differed in femoral tooth angle. Therefore, the exuviae of all four cicada species can be easily distinguished based on the morphological characters used in this study.

Final instars of cicada typically emerge from the ground and metamorphose into adults. Exuviae are typically left on tree branches or leaves where metamorphosis takes place. We investigated morphological characteristics of final instar exuviae for the most prevalent species of cicada that occur in urban areas of central Korea: Cryptotympana dubia, Meimuna opalifera, Oncotympana fuscata, and Graptopsaltria nigrofuscata. The characters analyzed were body length, body width, distance between compound eyes, proboscis length, antennal segmentation, femoral structure, and body coloration. The results of Principal Component Analysis for body-size related characters, body length, body width, distance between compound eyes, and proboscis length, revealed that only the first component (PCA1) was significant. The distribution of PCA1 showed that there were three non-overlapping groups: C. dubia, a complex of O. fuscata and G. nigrofuscata, and M. opalifera. Although O. fuscata and G. nigrofuscata were not distinguishable based on size-related characters, these two species differed in femoral structure. That is, the angle between the femur and femoral claw were generally 120° in G. nigrofuscata, whereas it was usually 90° in the other three species. Exuviae of M. opalifera were pale brown and non-polishing, whereas exuviae of three other species were all brown and highly polishing. Therefor, the exuviae of four cicada species can be easily distinguishable based on body size, femoral structure, and body coloration.

꿀벌부채명나방 종령유충의 whole body에서 gel filtration 방법으로 유약호르몬 결합 단백질을 분리, 정제하였다. 분리된 단백질은 column chromatography법과 전기영동법에 의해 등가성을 확인하였다. 이 결합단백질은 전기 영동법에 의해 32K, gel filtration 에 의해 28K의 상대적 분자량을 나타냈다. 또한, JH III에 대한 해리도는 3.9M로 확인되었다.