Simultaneous time monitoring observations of H2O 616 − 523, SiO J = 1–0, 2–1, 3–2, and 29SiO v = 0, J = 1–0 lines are carried out in the direction of the Mira variable star TX Cam with the Korean VLBI Network single dish radio telescopes. For the first time, the H2O maser emission from TX Cam is detected near the stellar velocity at five epochs from April 10, 2013 (Ø = 3.13) to June 4, 2014 (Ø = 3.89) including minimum optical phases. The intensities of H2O masers are very weak compared to SiO masers. The variation of peak antenna temperature ratios among SiO v = 1, J = 1–0, J = 2–1, and J = 3–2 masers is investigated according to their phases. The shift of peak velocities of H2O and SiO masers with respect to the stellar velocity is also investigated according to observed optical phases. The H2O maser emission occurs around the stellar velocity during our monitoring interval. On the other hand, the peak velocities of SiO masers show a spread compared to the stellar velocity. The peak velocities of SiO J = 2–1, and J = 3–2 masers show a smaller spread with respect to the stellar velocity than those of SiO J = 1–0 masers. These simultaneous observations of multi-frequencies will provide a good constraint for maser pumping models and a good probe for investigating the stellar atmosphere and envelope according to their different excitation conditions.

We report for the first time the occurrence of DWV-infected bumble bees (Bombus ignitus). For the present study, the detection of DWV virus from the female and male bumble bee was investigated in the same colony. The Deformed wing virus (DWV) of honeybee (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. Using specific RT-PCR protocols for the detection of DWV followed by sequencing of the PCR products we could demonstrate that the bumble bees were indeed infected with DWV. The virus was detected from Bombus ignitus, and its partial DWV gene was cloned and sequenced. The partial DWV gene encoding the polyprotein is 711-nt of 235 amino acid residues. The deduced nucleotide sequence of the polyprotein partial gene of DWV showed 96.9%, 96.2%, 96.8%, and 96.5% homology to other structure polyprotein partial gene of DWV from insects, respectively. Phylogenetic analysis further conformed that the deduced nucleotide sequence of the polyprotein partial gene of DWV divided to the outside tree. We describe the first time that presence of Deformed wing virus(DWV) from bumble bee(Bombus terrestris) in korea using RT-PCR.