To find best humidity condition for the use of Beauveria ARP14 against Grapholita molesta (Busck), first instar of G. molesta was treated with three conidial concentrations (1×107, 1×108, and 1×109 conidia/ml) in three different relative humidities (RH; 55, 75, and 95%). Mortality varied depending on concentration and humidity. Mortality in 95% RH was 1.5 and 2.7 times higher than 75 and 52% humidity at 1×109 conidia/ml and 16 hours after the exposure. However, the mycosis rates after 14 days were all not different among the treatments. In conclusion B. bassiana ARP14 could be most effective when applied higher at RH condition.

Among the Coleoptera, case bearing is found in four related subfamilies of the Chrysomelidae, the so-called Camptosomates: Clytrinae, Cryptocephalinae, Chlamisinae, and Lamprosomatinae (Böving and Craighead, 1931). Adults of casebearing chrysomelids feed on foliage of a variety of eudicots (Erber, 1988), but their larvae often show departures from strict phytophagy. Besides those with true herbivore larvae, that feed on green plant parts and complete development on the host plant, the larvae of most species live on the ground, in leaf litter, and feed on dry vegetable material and detritus (Erber, 1988; Brown and Funk, 2005). The larvae of some clytrine species live in ant nests (Erber, 1988; Brothers et al., 2000), but besides these mirmecophiles, many other clytrine larvae live in leaf litter and some few others on plants (Erber, 1988; Jolivet, 1988). The genus Smaragdina Chevrolat is distributed in the Paleoarctic region, and in the Oriental region (Chûjô & Kimoto, 1961; Erber & Medvedev, 1999; Gök, 2003; Maican, 2005, 2006). Forth-three Smaragdina species are distributed throughout northeast Asia, from Korea to Japan (Gressitt & Kimoto, 1961; Kimoto & Takizawa, 1994, 1997). In Korea a total of 5 species were known by Lee and An (2001). Recently, Smaragdina nipponensis Chûjô is newly recorded by Park et al. (2011). However, for the clytrine larvae has never been studied from Korea. The present study contains the description of the egg and first instar larva of S. nipponensis with notes on biology of adults and larvae, observes in natural conditions and in laboratory.