본 연구는 감꽃의 주요 향기성분을 알아보기 위해서 감나무 수령별로 감꽃의 향기성분을 SPME를 이용하여 분석하고, 기관을 꽃과 꽃받침으로 분류하여 향기성분을 비교하였다. 감나무 수령별 감꽃의 만개율은 15년생 이상, 10-14년생, 5-9년생 순으로 수령이 오래될수록 초기 만개율이 높았다. 감나무 수령에 따른 감꽃 향기성분 분석 시 동정된 주요 향기 성분은 a-pinene, butene, caryophyllene, cubebene, lavandulol, D-limoneneylangene, ylangene 등의 성분을 얻을 수 있었다. 대부분이 green 향, fruit계, floral계의 옅은 향도 포함하고 있었다. 감꽃에 존재하는 휘발성 향기성분의 수는 5-9년생은 30종, 10-14년생은 24종, 그리고 15년생 이상에서는 32종으로 수령이 5년 미만과 15년 이상에서 많은 향기성분이 조사되었다. 감꽃을 꽃받침과 나누어 향기성분을 비교하면 단감 ‘부유’ 품종 중 꽃의 향기 성분은 10개이고 상대적인 총 함량은 26.35%이며, 꽃받침은 향기 성분은 14개 이고 상대적인 총 함량은 46.28%로 꽃에 비해서 더 다양한 향기성분이 존재하는 것으로 조사되었다. 반면 떫 은감 ‘둥시’ 품종은 꽃에서는 6개의 향기성분이 17.58%, 꽃받침에서는 9개의 향기 성분이 50.27%로, ‘부유’ 품종 에 좀더 다양한 향기성분이 존재하였다. 본 연구는 감꽃 향기를 이용하고자 하는 향기산업에 기초자료를 제공하 는데 기여할 것이다.

국내 도입된 하이부쉬 블루베리의 품종 선발의 기초 자료로 활용하기 위하여 블루베리의 형태적 및 품종별 화기 특성에 관 해 연구하였다. 블루베리의 수술의 형태를 보면 화사는 판형이 었고 모용이 잘 발달되어 있으며, 개약 시 약벽이 열개되지 않고 돌출된 두 개의 관으로 꽃가루가 배출되어 독특하였다. 화분은 3월 하순경에 감수 분열된 후에 분리되지 않은 채로 성숙한 사분 자 화분(tetrad pollen)이었다. 약당 사분자 화분립 수는 400~ 1,300개였으며, 화분립 수가 많은 품종이 발아율도 높은 편이었 다. 블루베리의 암술은 5개의 심피로 구성되어 있으며, 화주는 갈라진 부분이 없이 하나였다. 자방당 배주 수는 39~67개 정도 였으며, 각 품종별로 변이 계수가 11.6~31.0%로 큰 편이었다. ‘Bluejay’와 ‘Sharpblue’간의 자가 및 타가교배 결과 자가교배보 다 타가교배에서 종자 형성률이 높았다.

UNUSUAL FLORAL ORGAN (UFO), a novel gene, is involved in controlling flowering initiation and development. In Arabidopsis, UFO is required for floral organ identity in the second and third whorls. However, the mode of expression and function of TaUFO have not been studied yet. The cDNA sequence of TaUFO is comprised of 1344 bp open reading frame which encodes 50.82 KDa polypeptide consisting amino acid residues. F-box protein, the components of TaUFO, plays an important regulatory role in a wide diversity of developmental and physiological responses. In almost all F box proteins, the N terminus of the protein contains the F-box motif, and the rest of the protein contains the protein-protein interaction domains required for target protein binding. In order to elucidate the function of the TaUFO, various phytohormones and abiotic stresses were applied on young seedlings (14 day after germination) and its transcripts were evaluated. TaUFO:GFP fusion construct was transformed into onion epidermal cells by particle bombardment to elucidate the subcellular localization of the TaUFO protein. The function of the F-box protein is to interact with target proteins. With the use of a yeast two-hybrid screen to isolate proteins interacting with the TaUFO (F box protein), we identified potential TaUFO interactive protein in wheat spikelet library.

Organ abscission is a programmed cell separation process that results in the detachment of an entire organ from a plant. Our goal is to understand the signaling pathway that regulates this physiological process. The receptor-like protein kinase, HAESA (HAE), and its paralog, HAESA-like 2 (HSL2), are both expressed in the floral abscission zones in Arabidopsis thaliana. Loss-of-function analyses of either gene do not show any phenotypical change, but the hae hsl2 double mutant shows an abscission-defect phenotype. Examination of the abscission zone by light and scanning electron microscopy showed that the abscission zone in the hae hsl2 appears structurally normal. The force required to remove the petals in wild type and hae hsl2 flowers was measured using a petal breakstrength meter. The force required to remove petals from the hae hsl2flowers at all stages of development was similar to that of wild type flowers that have not yet begun to abscise their petals. Taken together, these data support the role of HAE and HSL2 in the activation of cell separation, rather than differentiation of the abscission zone. Ethylene is also known to promote abscission; therefore we tested the ethylene-induced triple response and the effect of exogenous treatment on floral organ in the hae hsl2, revealing that HAE and HSL2 act independently of ethylene. This implies that the HAE is critical for floral abscission in concert with the action of HSL2.

To find out the relationship between pod development and cytokinin contents during reproductive stage of peanut, the cytokinin contents, trans-zeatin ribo-side (t-ZR) and dihydrozeatin riboside (diZR), were investigated at 0, 7, 14, 21 and 28 days after flowering (DAF). The amounts of t-ZR and diZR in cotyledon and first branch among primary branches were 3, 448 pmol/g (FW) and 4, 824 p/g (FW), respectively, which were higher than those of other branches. The t-ZR and diZR contents of lower parts on the branch from cotyledon node at 7 DAF were 579 pmol/g (FW) and 2, 028 pmol/g (FW), respectively, which were higher than those of upper parts. The cytokinin contents of reproductive organs as flowering progressed were increased at 0 and 14 DAF on branch and position of node. The cytokinin contents of upper part with pruning the lower part on the branch from cotyledon node were high 112-337% at 7 DAF and 14 DAF compared with those of the control. In case of remove the upper part of the first internode on main axis, t-ZR contents was 4.7 times higher than diZR contents at 7 DAF. The pod setting rate of flower and position on the branch from cotyledon node was closely related to the cytokinin contents during floral reproductive stage.