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        검색결과 6

        1.
        2018.11 구독 인증기관·개인회원 무료
        The porcine zygotic genome activation occurs along with global epigenetic remolding at the 4-cell stage. The histone acetylation, regulating DNA transcription, replication and so on, requires adequate acetyl-CoA. Acetyl-CoA produced by translocated pyruvate dehydrogenase in the nucleus of mammalian cells has been reported, which is commonly considered locating in the mitochondria. To find out whether the nuclear pyruvate dehydrogenase regulating the histone acetylation by controlling generation of acetyl-CoA, a multiple sgRNAs-CRISPR/Cas9 targeting strategy was employed to generate a pyruvate dehydrogenase E1 alpha1 (Pdha1) knockout (KO) parthenogenetic embryo model. Results showed that the targeting efficiency of Pdha1 reached more than 90%. Hence, this model was used in the subsequent experiments. Furthermore, a translocation of Pdha1 during zygotic genome activation was found by immunofluorescent staining and was significantly inhibited by Pdha1 KO. Meanwhile, the 8-cell stage embryo rate significantly decreased after 72 h (24.19% vs 12.53%, control vs Pdha1 KO), indicating a 4-cell arrest. In addition, the nuclear histone acetylation level significantly decreased when Pdha1 was KO. To determine whether the zygotic genome transcription was affected, the qPCR was performed and showed that the mRNA level of Eif1A, Acly, Sqle and Pdha1 all dropped significantly in the Pdha1 KO group compared to the control. In conclusion, the translocated Pdha1 generates acetyl-CoA for histone acetylation inside the nucleus of porcine embryos, which promotes the zygotic genome activation of porcine embryos.
        2.
        2014.04 구독 인증기관 무료, 개인회원 유료
        Embryonic genome activation (EGA) is a highly complex phenomenon that is controlled at various levels. New studies have ascertained some molecular mechanisms that control EGA in several species; it is apparent that these same mechanisms regulate EGA in all species. Protein phosphorylation, DNA methylation and histone modification regulate transcriptional activities, and mechanisms such as ubiquitination, SUMOylation and microRNAs post-tran-scriptionally regulate development. Each of these regulations is highly dynamic in the early embryo. A better under-standing of these regulatory strategies can provide the possibility to improve the reproductive properties in mammals such as pigs, to develop methods of generating high-quality embryos in vitro, and to find markers for selecting de-velopmentally competent embryos.
        4,300원
        4.
        2012.06 구독 인증기관·개인회원 무료
        Live offspring is obtained from in vitro production of porcine embryos, but the procedure is still associated with great inefficiencies. In mammalian oocytes, acquisition of meiotic competence coincides with a decrease in general transcriptional activity at the end of the oocyte growth phase. In this study, we investigated the expression and sub-cellular localization of positive transcription elongation factor P-TEFb (CDK9/Cyclin T1), a RNA polymerase II CTD kinase during pig oocyte growth and early embryonic development. Localization and expression of components involved in mRNA and rRNA transcription were assessed by immunocytochemistry in growing and fully-grown oocytes. In addition, meiotic resumption, germinal vesicle breakdown, nuclear transcription and embryonic genome activation (EGA) were analyzed in oocytes and embryos cultured in presence of a potent CDK9 inhibitor, flavopiridol. Our analyses, demonstrated that CDK9 became co- localized partially with phosphorylated Pol II CTD and mRNA splicing complexes. Surprisingly, CDK9 was co-localized with Pol I-specific transcription factor, UBF, and gradually localized in nucleolar peripheries at the final steps of oocyte growth. Later, CDK9 became associated with nucleolar structures at 4-cell stage. Treatment with flavopiridol resulted in arrest in meiotic resumption, germinal vesicle breakdown as well as a decline in global transcription. Flavopiridol also inhibited embryo development beyond EGA. All together, these data suggest that CDK9 has a dual role in both Pol I- and Pol II-dependent transcription in pig oocyte growth and embryonic development.