In order to establish symbiotic host-bacterial relationships, symbionts in insects evolved a mechanism to overcome host immune responses. Here we provide the resistance of symbiotic bacteria on the insect immune system. As a result, through the transposon mutagenesis, we found a salivary gland (SG) susceptible mutant. The disrupted gene was identified as nlpB involved in lipoprotein synthesis. The nlpB, bla double deletion mutant was sensitive to SG like nlpB-Tn5 inserted mutant. This mutant increases outer membrane permeability. It provides an explanation for SG susceptibility, because the antimicrobial peptide in SG would be able to translocate across the outer membrane more easily than in the wild type. These results indicate that nlpB and bla are likely to be important factors in terms of determining resistance against SG of Riptortus that is connected with the successful colonization of the Riptortus midgut.

Equine chorionic gonadotropin (eCG) is a heavily glycosylated glycoprotein composed of non-covalently linked α- and β-subunits. To study the function and signal transduction of tethered recombinant-eCG (rec-eCG), a single chain eCG molecule was constructed, and the rec-eCG protein was prepared. In this study, we constructed 5 mutants (Δ1, Δ2, Δ3, Δ4, and Δ5) of rec-eCG using data about known glycoprotein hormones to analyze the role of specific follicle stimulating homone (FSH)-like activity. Three amino acids of certain specific sites were replaced with alanine. The expression vectors were transfected into CHO cells and subjected to G418 selection for 2～3 weeks. The media were collected and the quantity of secreted tethered rec-eCGs was quantified by ELISA. The LH- and FSH-like activities were assayed in terms of cAMP production by rat LH/CG and rat FSH receptors. Then, the metabolic clearance rate analyzed by the injection of rec-eCG (5 IU) into the tail vein was analyzed. The mutant eCGs (Δ1, Δ4, and Δ5) were transcripted, but not translated into proteins. Rec-eCG Δ2 was secreted in much lower amounts than the wild type. Only the rec-eCG Δ3 (β-subunit: Gln94-Ile95-Lys96→Ala94-Ala95-Ala96) was efficiently secreted. Although activity is low, its LH-like activity was similar to that of tethered eCGβα. However, the FSH-like activity of rec-eCGβαΔ3 was completely flat. The result of the analysis of the metabolic clearance rate shoed the persistence of the mutant in the blood until 4 hours after the injection. After then, it almost disappeared at 8 hours. Taken together, these data suggest that 94～96 amino acid sequences in eCG β-subunit appear to be of utmost importance for signal transduction of the FSH receptor.

목적 : 본 연구는 김해지역 노인들의 가정내 일상생활활동 중 어려운 활동과 중요한 활동을 알아보고 성별, 연령별 차이를 알아보고자 하였다. 연구방법 : 연구 대상은 김해시에 거주하는 65세 이상의 재가 노인 49명을 대상으로 하였다. 연구도구는 구조화된 설문지를 사용하였다. 가정내 일상생활활동 중 어려운 활동과 중요한 활동에 대한 설문내용은 Occupational therapy practice framework domain and process(2002)와 일상생활활동평가도구를 기초로 하여 개발하였다. 설문조사 방법은 개별 면접으로 실시하였다. 결과 : 김해 지역 노인들의 일상생활활동 중 어려운 활동은 청소하기, 건강관리, 식사준비하기, 목욕하기 및 샤워하기, 기능적 이동과 세탁하기 순으로 나타났다. 중요한 활동은 건강관리, 식사하기, 개인위생, 대소변 조절, 목욕하기 및 샤워하기의 순으로 나타났다. 어려운 활동에서 성별에 따라 유의한 차이를 보인 활동은 식사준비하기, 대소변 조절 그리고 청소하기였으며, 연령에 따라 유의한 차이를 보인 활동은 개인위생이었다. 중요한 활동에서 성별에 따라 유의한 차이를 보인 활동은 식사준비하기였으며, 연령에 따라 유의한 차이를 보인 활동은 개인위생과 세탁하기이었다. 결론 : 본 연구결과를 통해 김해지역 노인들의 일상생활에서 어려운 활동과 중요 활동을 알 수 있었으며, 성별과 연령에 따라 활동에 차이가 있음을 알 수 있었다. 앞으로 노인을 대상으로 작업치료를 적용하거나 노인을 위한 생활기기 개발시 노인의 관점에서 남․여 또는 연령에 따라 어려운 활동과 중요한 활동을 고려하여 접근하여야 할 것이다.