본 연구는 최적의 당화 조건을 구명하기 위해 양파즙과 양파박을 0, 10, 20%로 첨가했다. 처리별로 25, 30, 35℃에서 72시간 동안 당화시켰다. 35℃의 당화 온도에서 처리 24시간 후 양파즙 첨가 20%에서 13.2 ◦Brix, 양파박 첨가 20%에서 19.6 ◦Brix의 당도를 나타내었다. 그러나 관능 평가결과 처리 간에 크게 차이가 나지 않았다. 양파박을 사용한 양파 당화물이 염증 유발물질인 히스타민 방출량을 14% 억제하는 효과를 나타내었다. ELISA 분석 결과 양파당화물이 대조군에 비해 IL-8과 TNF의 방출을 보다 효과적으로 차단할 수 있었다.

In vitro fermentation was conducted to figure out alternative fiber sources for horse feed. For the development of value-added products as a horse feed resource, the pomaces from apple, carrot, grape, and citrus were fermented under solid-state conditions in the presence of 60% soybean meal with 40% of each fruit pomace at 60% of moisture content. Lactobacillus plantarum SK3873, Lactobacillus plantarum SK3893, Weissella cibaria SK3880, and Bacillus subtilis SK3889 were isolated from the fermented fruit pomace by inoculation of horse feces. For the growth of Bacillus subtilis, Saccharomyces cerevisiae, and Lactobacillus plantarum, they were inoculated in 3-step order at 0, 12, and 24 h, respectively. The fruit pomace was fermented for 48 h at 35°C. The pH of the apple, carrot, grape, citrus and all mixed pomaces decreased from 5.45~6.25 to 4.40~4.77. Microbial growth was maintained at 108~109 cfu/g. After 12 and 24 h incubation, dry matter of carrot pomace were highest at 54.84 and 56.66%, respectively (P<0.05) and that of grape pomace was lower than others during fermentation (P<0.05). Dry matter was generally reduced by about 20%. NDF decreased gradually or maintained after 24 h, indicating the fiber degradation. Ash content tended to decrease during fermentation. After 48 hours fermentation, Bacillus, yeast and Lactobacillus showed an excellent growth by using juice by-products. These results suggest that fermented juice pomace has a potential as horse feedstuff with probiotics to maintain beneficial microflora in horse gut.