The recent prosthetic technologies pursue to control multi-DOFs (degrees-of-freedom) hand and wrist. However, challenges such as high cost, wear-ability, and motion intent recognition for feedback control still remain for the use in daily living activities. The paper proposes a multi-channel knit band sensor to worn easily for surface EMG-based prosthetic control. The knitted electrodes were fabricated with conductive yarn, and the band except the electrodes are knitted using non-conductive yarn which has moisture wicking property. Two types of the knit bands are fabricated such as sixteen-electrodes for eight-channels and thirty-two electrodes for sixteen-channels. In order to substantiate the performance of the biopotential signal acquisition, several experiments are conducted. Signal to noise ratio (SNR) value of the knit band sensor was 18.48 dB. According to various forearm motions including hand and wrist, sixteen-channels EMG signals could be clearly distinguishable. In addition, the pattern recognition performance to control myoelectric prosthesis was verified in that overall classification accuracy of the RMS (root mean squares) filtered EMG signals (97.84%) was higher than that of the raw EMG signals (87.06%).