This study was conducted to investigate the effects of Kojongsi persimmon peels as a mushroom substrate on antioxidant activity of Pleurotus eryngii. The Pleurotus eryngii was growth on the medium with 0, 5, 10, 15, 20, 30, 40, 50% Kojongsi persimmon peels. The Pleurotus eryngii was extracted into ethanol and their antioxidant activities were analyzed. The total polyphenol content was highest in the 20% Kojongsi persimmon peels treatment (40±0.38 mg of gallic acid equivalents/100 g) than 0% Kojongsi persimmon peels treatment (21 ±0.18 mg of gallic acid equivalents/100 g). The 20% Kojongsi persimmon peels treatment showed better DPPH scavenging activity (36.81±0.23% at 1 mg/ml), ABTS scavenging activity (39.03±0.10% at 1 mg/ml) and reducing power (0.3±0.10% at 1 mg/ml) than 0% Kojongsi persimmon peels treatment and another extract ones. Overall, total polyphenol content and antioxidant activities of extracts from Pleurotus eryngii was increased dose dependently and 20% Kojongsi persimmon peels treatment was showed better total polyphenol content and antioxidant activities than 0% Kojongsi persimmon peels treatment and another extract ones.

The Kojongsi persimmon peels is byproduct after production of dried persimmon. This study was conducted to investigate optimal mixing ratio of Kojonsi persimmon peels as a substrate for cultivation of Pleurotus eryngii. The primary nutritive material of substrate for Pleurotus eryngii was corncob, soybean meal, beet pulp, ground corn and bran. Five to forty percent of persimmon peels were mixed with primary nutritive materials as new substrate. The chemical properties of substrate mixed with Kojongsi persimmon peels was similar to the mixture without Kojongsi persimmon peels in T-C, T-N, C/N ratio, and other nutrients. Mycelial growth time on medium with 5 to 30% persimmon peels was similar to the control without persimmon peels as 27 days but that on medium with 40% and 50% persimmon peels was delayed for 10 days. The time of pinhead formations in medium with 5 to 30% persimmon peels was 12 days, the growth time to harvest was 6 days. These results were similar to those of the control without persimmon peels. The sizes of pileus of treatments with 5 to 30% persimmon peels were tend to be smaller compared with the control. The length of stipe of 10% persimmon peels treatment was 80 mm which was shorter than that of the control with 120 mm.

본 연구는 지역 특산물인 감과피를 이용한 버섯배지 개 발을 위한 기초자료로서 감과피의 이용 가능성을 검토하 고자 수행되었으며 감과피는 경상남도 산청지역에서 곶감 을 생산하고 남은 것을 수거하여 건조한 후 사용하였고 공시균주는 ASI 2312를 사용하였다. 대조구는 혼합배지:콘코브:톱밥(220:65:15, v/v)을 혼합한 기본배지를 사용하 였고 기본배지 중 혼합배지를 5%, 10%, 15%, 20%, 30%, 40%, 50%의 건조된 감과피 처리구로 대체한 처리 구를 시험구로 사용하였다. 대조구인 기본배지에 비해 시 험구인 5%, 10%, 15%, 20%, 30%, 40%, 50% 감과피 처 리구의 T-N 함량은 감과피 첨가량이 증가할수록 감소하 는 경향을 나타내었으며 T-C 함량은 큰 차이가 없었고 C/ N율은 감과피 첨가량이 증가할수록 증가하는 경향을 나 타내었다. 감과피 첨가량이 균사생장에 미치는 영향을 조 사한 결과 대조구보다 감과피가 첨가된 모든 시험구에서 균사생장이 느리게 나타났다. 감과피 첨가량에 따른 배양 일수는 대조구인 혼합배지에 비해 감과피를 30%까지 첨 가하여도 배양일수와 초발이 소요일수에는 뚜렷한 차이는 없었지만 감과피 첨가량이 증가할수록 배양일이 길어지는 경향을 보였으며 혼합배지에 비해 감과피 첨가비율이 증 가할수록 갓의 직경은 감소하고 대의 길이는 길어지고 직 경은 감소하는 경향을 나타내었다. 수확 후 동결 건조한 큰느타리버섯의 수분, 단백질, 조회분 함량은 대조구와 시 험구에서 유의성 있는 차이를 나타내지 않았지만 조지방 함량은 감과피 첨가량이 증가함에 따라 증가하는 경향을 나타내었고 β-glucan 함량은 대조구에 비해 10%와 15% 감과피 첨가구에서 높게 나타났다. 따라서 대조구와 비교 했을 때 15% 감과피 첨가구는 자실체 수량도 높게 나타 나고 β-glucan 함량도 높게 나타났기때문에 감과피 첨가 비율은 15%가 가장 적합한 것으로 사료된다.