본 연구는 DFT 재배 시스템을 이용한 밀폐형 식물생산시스 템에 MB처리 유무에 따른 상추(Lactuca sativa L.)와 배초향 (Agastache rugosa Kuntze)의 생육과 양액 특성을 조사하기 위해 수행되었다. 상추와 배초향은 밀폐형 식물생산시스템에 서 23일간 재배되었다. MB는 매일 9시, 13시, 17시에 5분씩 16일간 처리하였다. 상추와 배초향의 엽장, 엽폭, 엽면적, 생 체중 및 건물중은 MB처리구에서 대조구에 비해 유의하게 낮 았다. 상추와 배초향의 총 뿌리길이, 뿌리 표면적, 근단 수도 MB처리구에서 유의적으로 낮게 나타났다. 평균 뿌리 직경의 경우, 상추는 처리 간에 차이가 없었으나, 배초향은 MB처리 구에서 유의적으로 굵어 종 간에 차이가 있음을 확인하였다. 본 연구 결과, DFT 방식으로 상추와 배초향을 재배할 때 MB 처리는 비생물적 스트레스를 유발하여 생육을 억제하는 것으 로 나타났다.

Protoplasts were isolated from the primary leaves of lettuce (Lactuca sativa L.) seedlings 10 days after in vitro germination. The leaves were stripped and incubated in an enzyme mixture consisting of 1.2% Cellulase R-10 and 0.3% Macerozyme R-10 in cell and protoplast washing solution (CPW) overnight. The average protoplast yield was 8.25 x 106 protoplasts per g of fresh leaf tissue. When protoplasts were cultured at a density of 3.0 × 105 protoplasts/mL in agarose solid KM8P/KM8 medium, first and second divisions were observed in the protoplasts within a week. Protoplast-derived microcolonies formed after 4 weeks of culture, and visible colonies were present after 3 months of culture. Protoplast-derived microcalli were transferred to Murashige and Skoog medium supplemented with 2.0 mg/L kinetin and 0.1 mg/L NAA and incubated in the light for 3 weeks. They grew into callus, which then regenerated into plants after 7 weeks of culture. The regenerated plants grew as apparently normal flowering fertile plants.

Three benzimidazole pesticides commonly used in korean lettuce were subjected to a field residue trial to ensure safety of terminal residue in the harvest. The residual patterns of three benzimidazole pesticides, which were carbendazim, benomyl and thiophanate-methyl were examined after applying with the recommended dose in two types of korean lettuce (Chima and Chuckmeon) and their DT50 were calculated. In Chima lettuce, biological half-lives of carbendazim, benomyl and thiophanate-methyl were 2.56, 1.37 and 2.54 days, respectively and their required time under MRL(5.0 mg/kg as carbendazim) were 4.5, 2.2 and 1.0days. In Chuckmeon lettuce, biological half-lives of them were 3.41, 1.70 and 4.20 days, respectively and their required time under MRL were 5.4, 1.9 and 0.5days.