The purpose of this study was to investigate the effects of a repetitive lifting task on the level of activation and median frequency of the paraspinal muscles, and to provide basic data of the maximal acceptable duration of the lifting task to avoid muscle fatigue. Ten healthy male subjects were recruited as participants and they repetitively (12 lifts/min) lifted a box (46 ㎝ × 30 ㎝ × 30 ㎝, 15 kg) for 10 minutes. Electromyographic data (muscle activation and median frequency), heart rate, and Borg CR10 score were recorded at 1, 3, 5, 7, and 9 minutes after the lifting task. Electromyographic data was recorded from the elector spinalis, mutifidus, external oblique abdominis, and rectus abdominis for 1 minute. The results showed that as the repetitive lifting task progressed, the heart rate and Borg CR10 score significantly increased. In addition, activation of the muscles increased. The median frequency significantly decreased over time in the elector spinalis, mutifidus, and external oblique abdominis (right side), except for the external oblique abdominis (left side) and rectus abdominis. It is suggested that the median frequency recorded from a dynamic task is used to monitor muscle fatigue. Furthermore, the repetitive lifting task (15 kg, 12 lifts/min) should not continue for more than 3 minutes in order to avoid muscle fatigue.