The BIG11003 strain was isolated as protein-degrading actinomycetes from cucumber farm. The effect of plant natural resources (Azadirachta indica L and Sophora angustifolia) of juveniles and the hatching of eggs of Meloidogyne sp. were examined the laboratory. The nematicides (fosthiazate), actinomycetes protease and S. angustifolia extract resulted in 45.6, 46.6 and 51.23% mortality of J2 of M. incognita. The nematicides (fosthiazate), A. indica L and S. angustifolia extract resulted in 2.06, 3.96 and 6.22% hatch of egg of M. incognita. Antinematode activity test had done after the formulation of liquid (Nemastar) with mixture of additives and inerts into selected highly active natural material and actinomycetes protease. In pot and field experiments, the nemastar had good control efficacy against plant root-knot nematodes. Anti-nematode activity of strain BIG11003 from phylogentic analysis based on 16S rDNA sequences, it is suggested the Streptomyces koyangensis.

Root-knot nematode damage was found on yam, Dioscorea bulbifera in Andong Korea. From the root-knots, female nematodes were isolated and subjected to DNA sequence analysis. Sequence of cytochrome oxidase subunit Ⅱ (COII) was analyzed from the genomic DNA of the isolate. COII locus size and sequence of the nematode isolate were similar to those of Meloidogyne javanica or M. incognita. However, an analysis of HinfⅠ restriction site, a species-specific character between these two species, showed that the isolate did not match to either M. javanica or M. incognita.