This study was conducted to investigate the influence of moisture removal using a moisture condensation tube on the odor concentration, when sampling a malodorous substance from an odor discharge facility’s emission sites. For high-temperature and humid gas streams, the odor concentration was decreased through the use of a moisture condensation tube. The multiple odor concentration of the high-temperature and humid gas streams emitted from boiler-burning equipment decreased from a 3,000 to a 1,221 dilution factor when using one or two moisture condensation tube. This multiple odor concentration was further decreased to a 1,000 dilution factor by using two moisture condensation tubes and glass beads, and also was decreased to a 374 dilution factor by using two moisture condensation tubes and silicagel. Among the designated offensive odorous substances, ammonia, trimethylamine and acetaldehyde that have high solubility in water showed high reduction rate of their concentration. Compared to the result using a sampling tube only, the concentration was decreased by 94.8% ~ 97.7% for ammonia, by 87.5% ~ 95.9% for trimethylamine and by 100% for acetaldehyde. The findings of this study indicate that sampling using a moisture condensation tube affects the concentration of multiple odors. Therefore, it is considered that using a sampling tube only for emissions sampling enhances analytical accuracy and precision rather than using moisture condensation tube with sampling tube, even for the emissions containing moisture.