셀룰로오스는 지구상에서 가장 풍부하게 존재하는 재생 가능한 천연 다당류로서 glucose의 β-1,4 결합에 의하여 이루어진 물질이자 고등식물의 주요 구성성분으로서 현재 제지, 펼프 및 방적산업을 비롯한 다양한 분야에서 사용되고 있다. 셀룰로오스의 소비가 급증함에 따라 그 원료로 사용되는 목재에 대한 수요도 갈수록 높아지고 있으나 원료공급과 환경문제로 인하여 제지 대체물질에 대한 연구가 절실한 형편이다(Sutherland 1998). 따라

Pleurotus ostreatus 'Miso' is a mutant strain showing white color in pileus from the known parent strain 'Wonhyeong No. 1'. Shape and several other characters also vary with culture conditions. Mating experiments were performed to understand interstrain mating relationship using monokaryons of the parent and the mutant strains. All monokaryons were grown from single spores isolated from freshly collected fruit bodies. Pairings were performed in 90 mm petri dishes on PDA. They were allowed to grow at 25℃ until two fronts of the advancing mycelia met and developed a conspicuous contact zone. The contact zone and the outer edges of paired colonies on each plate were examined for clamp connections. The crosses were considered positive when clamp connections were at the growing margin of either side of the interacting monokaryons. The parent strain 'Wonhyeong No. 1' and the mutant strain 'Miso' resulted in tetrapolar incompatibility system in intrastrain crosses. Normal compatible matings could be visibly distinguished from incompatible ones by the rapid growth and gross morphology. The resultant dikaryotic colony was also confirmed microscopically by the presence of clamp connections from incompatible pairings. In interstrain crosses, each monokaryotic tester strains of the parent strain was out-crossed to monokaryotic tester strains of the mutant. The four classes of tester strains representing the four incompatibility types were chosen from each mutant and parent strains. As a result of these crosses it was found that both strains share the same A and B incompatibility factors yielding 25% compatibility.