구약에서 하나님의 택함을 받은 이스라엘이 가진 선교적 공동체로의 정체성은 이미 인간 창조 서술에 암시되어 있다. 그것으로부터 하나님의 선교가 먼저 인간의 선교(창 1:26 이하)로 그리고 이스라엘의 선교(창 12:3)로 발전하며, 점점 더 구체화되고 있다. 특히 이스라엘이 출애굽기에서 처음 민족적 단위로 세워지며, 온 세계 민족에 대한 선교적 공동체로서의 정체성을 지니게 되었음은 하나님의 백성의 공동체로 세워지는 출애굽기 19장 4-6절에 명시화되어 나타난다. 또한 레위기는 오경의 신학적 핵심에 서 있을 뿐 아니라 책 자체에 고안된 중앙 집중적 구조를 통해서도 자신의 백성의 공동체에 임재하며, 그들에게 속죄의 현재성을 지속적으로 보장할 뿐만 아니라, 자신의 백성을 통해 그 속죄의 은혜를 온 세상에까지 전파할 사명을 부여하는 하나님을 제시한다. 구약이 제시하는 이러한 하나님의 백성의 공동체가 가진 정체성은 신약의 교회에 직접적으로 연결될 뿐 아니라, 에베소서가 제시하는 교회의 정체성으로서의 통일성, 성결성 그리고 보편성에 맞닿아 있으며, 나아가 신약과 오늘날의 선교적 공동체로서의 교회가 가져야 할 모습을 규정하고 있다.
Totally, 26 collections, 17 from Korea and 9 from China, were investigated for their sequences of 5S rDNA, especially the non-transcribed spacers (NTSs). Sequences of 5S rDNA were isolated by PCR using the primers, 5s-rRNA1 and 5s-rRNA2. Genomic DNA PCR produced single amplification of 300, 330, or 350 base pair fragments. Sequence analysis revealed that all inserts contained the part of 5S rDNA gene sequence and the full length of the NTS region. Three different sizes of the fragments were confirmed due to different size of NTS and their length were 300bp, 330bp and 350bp, respectively. Among 17 Korean foxtail millets tested, 14 collections showed single 300bp amplification. Longest fragment amplification, 350bp, was obtained only from the foxtail millet from China origin, even though 2 of them include 300bp fragment. CLUSTALW multiple alignments of 26 foxtail millets clearly revealed 4 areas with certain degree of sequence heterogeneity (region I, II, III, IV). Among 4 boxed areas, foxtail millet genotypes from China have distinct insertion especially in region III. Five of them have extra insertion of sequence and their additional sequences were either 45 or 48 base pair. Three Korean foxtail millets have 32 bp insertion. Other 8 Korean collections have short insert sequences (6 to 8 bp), 3 with 8 bp and 5 with 6 bp. In addition to insert, deletion sequences were also confirmed as major deletion was observed in region II of Chinese collection. The size of deletion was 7 bp long. According to phylogenic tree constructed using MEGA4 program, clear grouping was not revealed. To obtain more convincing results various collections from many countries should be obtained and analyzed to distinguish different germplasm from different origin.
Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.