본 연구에서는 시판 메밀차 열수 추출물의 항산화 효과 및 신경세포 보호효과를 조사하였다. 시판 메밀차 열수 추출물의 ABTS 라디칼 소거 활성, FRAP 및 MDA 생성 저해 실험결과 농도 의존적인 경향이 나타났으며 또한 높은 항산화 활성을 보여주었다. 과산화수소로 유발된 산화적 손상에 의한 ROS 축적량을 조사한 결과 단독 처리구보다 메밀차 열수 추출물 처리구에서 낮은 ROS 축적량을 나타내었다. MTT 및 LDH 분석을 통한 PC12 세포

Caulis Bambusae in Taenia is widely used in Korea and China due to its various pharmacological activity. The present study aims to investigate the effect of the methanol extract of Caulis Bambusae in Taenia (CB) from Phyllostachys nigra Munro var. henonis Stapf (Gramineae) on amyloid β protein (25-35) (Aβ (25-35)), a synthetic 25-35 amyloid peptide, -induced neurotoxicity using cultured rat cortical neurons. CB, over a concentration range of 10-50μg/μl, inhibited the Aβ (25-35) (10 μM)-induced neuronal cell death, as assessed by a 3-[4,5-dimethyIthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. CB (50 μg/μl) inhibited glutamate release into medium induced by 10 μM Aβ, (25-35) which was measured by HPLC. Pretreatment of CB (50 μg/μl) inhibited 10μM Aβ (25-35)-induced elevation of cytosolic calcium concentration ([Ca2+]c), which was measured by a fluorescent dye, fluo-4 AM, and generation of reactive oxygen species. These results suggest that CB prevents Aβ (25-35)-induced neuronal ell damage in vitro.