This study aimed to evaluate the anti-inflammatory, whitening, and anti-wrinkle effects of nicotinamide mononucleotide (NMN) and NMN-containing serum. To investigate the anti-inflammatory effects of NMN, a lipopolysaccharide (LPS)-induced RAW264.7 macrophage model was used. The results demonstrated that NMN exhibited no cytotoxicity at concentration up to 1%. Additionally, 1% NMN significantly inhibited nitric oxide (NO) production induced by LPS and suppressed the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), both of which are key inflammatory enzymes. Furthermore, to assess the whitening and anti-wrinkle effects of NMN, tyrosinase inhibition, elastase inhibition, and collagenase inhibition assays were conducted. The results showed that 1% NMN inhibited tyrosinase activity by 10.85%, elastase activity by 18.69%, and collagenase activity by 91.94%. These research results suggest that although NMN has a relatively limited whitening effect, it has significant potential as an anti-inflammatory and wrinkle-improving agent, suggesting its potential as a cosmetic material.

Nicotinamide (NAM), a water-soluble derivative of vitamin B3, has emerged as a potential therapeutic agent for bonerelated disorders. In particular, it promotes bone metabolism and alleviates delayed tooth eruptions associated with cleidocranial dysplasia (CCD). NAM serves as a precursor for nicotinamide adenine dinucleotide, a key coenzyme involved in cellular metabolism that plays an essential role in oxidative phosphorylation and mitochondrial function. Recent research has highlighted the capacity of NAM to enhance osteogenic differentiation and regulate the interaction between osteoblasts and osteoclasts, which is critical for maintaining bone homeostasis. Moreover, the effect of NAM in preventing delayed tooth eruptions in CCD models underscores its potential as a noninvasive therapeutic option. Considering its safety profile and therapeutic potential, NAM is a promising candidate for longterm treatment of bone diseases and prevention of age-related bone disorders.