Currently, the development of evaluation technology for vibration and shock loads transmitted to spent nuclear fuel and structural integrity of spent nuclear fuel under normal conditions of transport is progressing in Korea by the present authors. Road transportation tests using surrogate spent nuclear fuel were performed in September, 2020 using a test model of KORAD-21 transportation cask and sea transportation tests were conducted from September 30 to October 4, 2021. In order to investigate amplification or attenuation characteristics, according to the load transfer path, a number of accelerometers were attached on a ship cargo hold, cradle, cask, canister, disk assembly, basket, and surrogate fuel assemblies and to investigate the durability of spent nuclear fuel rods, strain gages were attached on surrogate fuel assemblies. A ship named “JW STELLA” which has similar deadweight (5,000 ton) of existing spent nuclear fuel transportation ships was used for the sea transportation tests. The ship is propelled by 1,825 hp two main engines with two 4-bladed propellers. There are two major vibration sources in the ship. One is the vibration from waves and the other is the vibration from the engine and propeller system. The sensor locations on the ship were determined considering the vibration sources. The sea transportation test was performed for 5 days, the test data were measured successfully. The ship with the test model was departed from Changwon and sailed to Uljin, sailed west to Yeonggwang and then returned to Changwon. In addition to sailing on a designated test route, circulation test, braking/acceleration test, depth of water test, and rolling test were conducted. As a result of the preliminary data analysis of the sea test, power spectral densities and shock response spectrums were obtained according to the different test conditions. The vibratory loads caused by the wave mainly occurred in the frequency range of 0.1 to 0.3 Hz. The vibratory loads caused by the propeller occurred near the n/rev rotating frequencies, such as 5, 10, 20 Hz etc. However, those frequencies are far from the natural frequencies of local mode of the fuel rods, so it is considered that the vibratory loads from the wave and the propeller do not have a significant influence on the structural integrity of the fuel rods. Among all the test cases, maximum strain occurred at SG31 near the bottom nozzle on the test; the magnitude was 73.62 micro strain. Based on the analyzed road and sea transportation test data, a few input spectra for the shaker table test will be obtained and the shaker table test will be conducted in 2022. It is expected that the detailed vibration characteristics of the assembly which were difficult to identify from the test results can be investigated.