For the purpose of developing Korean Herbalogy with the plants of Oleceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigative and the results obtained were as follows: 1. According to sum of 101 species in Oleaceae family they were classified into Syringa genera 29, Ligustrum genera 24, Fraxinum genera 20, Osmanthus genera 20, Forsythia genera 8, Albeliophyllum genera 8. Thus it was noticed that Syringa genera was the main kind, some 29% in total. 2. There were totaled 19 genera and 101 species in Korea and among them modecinal plants are 6 genera, 28 species, in total but the number of species may be added because of similar plants. 3. According to the oriental name which can be used for medical purpose, the medicinal plants beloning to the Oleaceae fimily were classified as Fructus 3, Folium 5, Radix 4, Flos 3, Cortex 4, Lignum. Thus it was noticed that Folium was the Main kind. 4. According to the number of species of the origin plants about each chinese materia medicals, they were classified into FRUCTUS FORSYTHIAE 6, FRUCTUS LIGUSTRI 3, CORTEX FRXINI 2, FRUCTUS SYRINGA 3, FRUCTUS LIGUSTRUM OBTUSI FOLIO 2, FLOS JASMI NUDIFIORUM 1, FLOS JASMINI SANBAC 1. 5. According to nature and foavour of medicinal plants, they were classified into cold, cool ; 9, balance ; 6, wormth ; 3. Thus it was noticed that cold, cool is the main in nature and flavour of medicinal plants unidentified 3. 6. According to the Properties and Principal Curative action, they were classified into, clearing up heat and toxin 7, medicines for rehulating the flow of gi and allevating pain 3, resolving phlegm and cough, invigorating kidney and liver 2, clearing up heat and moisture, healthiness eleminating bloodstasis and aleviating pain, grgulating the flow of gi and invigorating blood circulation 1 each. 7. Comparing o whole medicinal plants 20 kinds, toxic durgs include minor toxin were 3 kinds, 7% of the whole. Thus toxic durgs were rare. From this result, it was revealed that the plants for medical purpose in Oleaceae was 28 kinds of the whole, in which Folium was mostly abundunt, were distributed (over) the whole country (widly) that it will be used for clinical treatments more easily. For (about) unidentifical drugs, it is considered that many experiments and clinical approaches must be continued.

Abeliophyllum distichum is a monotypic taxon of Oleaceae and endemic to Korea. A comprehensive study on embryogeny and fruit and seed coat ontogeny in Abeliophyllum was carried out via microtome and light microscopy. The fertilization occurs during mid– to late April and embryo matures by early July. The embryo development follows the general fashion from globular embryo – transition embryo – heart shaped embryo – torpedo embryo – walking-stick embryo to mature embryo. The pericarp clearly differentiates into three histological zones: exocarp, mesocarp, and endocarp. The young seed comprises 10-12 cells thick seed coat and the mature seed coat comprises an exotesta, 6-8 mesotesta and an endotesta. Any crystals, phenolic-like compounds, idioblasts, and the sclereids are not found in pericarp as well as seed coat. An overall development confirms Solanade type of embryogenesis in Abeliophyllum. The endocarp becomes more prominent in mature fruit

This experiments were carried out to find out the effects of different explant materials, kinds and concentration of plant growth regulators, and total nitrogen and sucrose contents on the in vitro regeneration of Abeliophyllum distichum Nakai. The effects of growth regulators on regeneration from 3 explant sources (leaf, internode and node) were more or less same. Leaf explants produced only callus with 2ip (Isopentenyladenine) and NAA (Naphthaleneacetic acid) treatment and other regulators had no effects. Test with internode explants yielded about same results but callus was obtained with 2,4-D (2,4-Dichlorophenoxyacetic acid). Node explants resulted in shoot regeneration by all regulator treatment except NAA and 2,4-D, but control also showed similar results. Callus formation from internode and node explants was vigorous by 2ip, zeatin, and 2,4-D treatments and high NAA concentration resulted in higher callus formation. In this experiment, various mixed treatment of growth regulators were also employed, using node as explant material. Shoot regeneration was obtained with BA (Benzyl adenine) + NAA treatments but the results were comparable with control. Generally shoot and root regeneration was poor with all combined treatment except 2ip + NAA and 2,4-D + NAA. However, callus was formed readily with all treatments. In this experiment, combined treatments of regulators were applied on the callus derived from singular regulator treatment. The results showed no shoot and root regeneration with any combination of 2,4-D, IAA (Indoleacetic acid) and NAA, but soft milky white callus was formed in all the treatments. No shoot and root regeneration was observed with any combination of 2iP, NAA and IAA, but somewhat hard, light green callus was formed in all the treatments. Callus formation decreased with high kinetin concentration in case of kinetin + NAA treatment. The experiments with total nitrogen content of media showed that low concentrations of 15 and 30mM were effective for the shoot and root regeneration. Sucrose experiment demonstrated shoot regeneration with 1~4% concentration, and root and callus formation with 2~4%. No root and callus formation was observed with 0 and 1% sucrose.

RAPD analysis was performed to discuss the taxonomic status and phylogenetic relationships among the tribe Forsythieae and related groups. Two hundred and eighteen scorable polymorphic bands were detected from fourteen oligonucleotide primers. From the results of RAPD analysis by Nei and Li's genetic distance, each individuals of Abeliophyllum distichum showed high genetic relationships with ranging from 0.085 to 0.301, also the genus Forsythia showed from 0.042 to 0.655 among the species and populations. But, Abeliophyllum and Forsythia showed distinct dissimilarity, ranging from 0.610 to 1.258. And genetic differences among the population of Forsythia were 0.042 in F. koreana, 0.275 in F. saxatilis, 0.275 in F. ovata, 0.279 in F. nakaii, and 0.249 in F. viridissima. The UPGMA phenogram of tribe Forsythieae based on the results of RAPD analysis were presented that Abeliophyllum is distinct genus different from Forsythia. NJ tree which applied as the outgroups Fontanesia and Jasminum was derived, and it showed that tribe Forsythieae might be a monophyletic group. The genus Fontanesia was showed as sister group of tribe Forsythieae. Among the populations of taxa in Forsythia, F. koreana and F. saxatilis were more closely related, and F. ovata and F. nakaii were very closely related to F.japonica. And Fontanesia was the sister group of tribe Forsythieae.