This study was conducted to investigate cryopreservation of pearl oyster, Pinctada fucata martensii larvae. Four cooling rates (-0.25, -0.5, -0.75 and -1.0/min.) were used to examine a proper cooling rate during cryopreservation of trochophores before seeding temperature (-12). Seven developmental stages (early and late trochophores, early and late D-shaped larvae and early, middle and late umbo stage larvae) and different sugars (fructose, glucose and sucrose) were used to investigate optimal larval stage and effective sugar in cryopreservation of larvae. The survival rates of frozen-thawed trochophores increased at cooling rate of -1.0/min. As larval developing, survival rate of frozen-thawed larvae increased, except umbo stage larvae, and especially late D-shaped larvae highly survived as 91%. Addition of sugar revealed positive effect on cryopreservation in this experiment and 0.2 M glucose and sucrose mixed with 2.0 M dimethyl sulfoxide significantly enhanced survival rate of larvae (P<0.05). The results of our study indicate that desirable cooling rate, developmental stages of larvae and effective sugar far cryopreservation of pearl oyster, P. fucata martensii larvae are -1/min, late D-shaped larvae and 0.2 M glucose and sucrose, respectively.

냉동보존이 진주조개(Pincata fucata martensii) 유생의 형태 및 구조에 미치는 영향을 알아보기 위하여 냉동전후 D형 및 각정기 유생을 광학 및 전자현미경으로 조사하였다. 동해방지제는 0.2 M sucrose를 첨가한 2.0 M 를 사용하였다. 냉동후 유생은 일부 패각이 손상되긴 했지만 hinge와 prodissoconch가 뚜렷하게 나타났으며, 소포체, 지질 과립, 미토콘드리아, 핵 등을 포함한 세포내 소기관들이 고르게 분포되어 있었다

우리나라에서 진주양식의 대상패류인 진주조개 (Pinctada fucata martensii) 담륜자를 냉동보존하기 위하여 4가지 동해방지제를 사용하여 냉동실험을 실시하고, 적합한 동해방지제를 찾고자 하였다. 동해방지제인 dimethyl sulfoxide (DMSO), ethylene glycol, glycerol과 1, 2-propanediol을 희석액인 0.2 M sucrose에 각각 0.01, 0.1, 1.0과 2.0 M이 되도록 혼합한 다음, 10