In this study, we investigated the amino acids content of shiitake mushrooms grown in eight different media and under three different temperature conditions. The sawdust media were supplemented with 4 kinds of nutrients - rice bran, barley, bean curd refuse, and red ginseng refuse in an 8:2 ratio with or without 0.5% calcium chloride, and the fruiting temperature was set to low (13–15oC), medium (18–20oC), or high temperatures (24–26oC). Seventeen amino acids were detected, and the content varied depending on the compositions of the media. The highest total amino acid content (209.7 mg/g) was measured in mushrooms from the media with red ginseng refuse, and the glutamic acid content was the highest (51.3 mg/g) in mushrooms from the media with barley. In terms of fruiting temperature, the amino acid content was the highest (38.2%) at medium temperature followed by the content at low (32.5%) and high temperature (29.3%). The amino acid composition of shiitake mushrooms was similar between the medium and low temperature conditions.

New shiitake (Lentinula edodes) strains “Dasanhyang”, “Chunjang-1ho” and “Poongnyunko” were breeded for bed-log cultivation. Fruit-body production of “Dasanhyang” was most at summer and autumn. The total amount of fruit-body production during 4 years(one generation) was 31kg/㎥ log. The fruit-body is hemispherical shape, brown colored and diameter of pileus is ca. 54 mm. Optimal temperature of fruit-body formation was 14~2 2℃, and the fruiting is concentrated. Fruit-body production of “Chunjang-1ho” was most at spring and autumn. Optimal temperature of fruit-body formation was 12~22℃ and range of fruiting temperature is broad. The diameter of pileus is ca. 46 mm. The fruiting is concentrated, and the total amount of fruit-body production during 4 years(one generation) was ca. 18kg/㎥ log. Fruit-body production of “Poongnyunko” was most at summer and autumn. Optimal temperature fruit-body formation was 17~24℃. It is high-temperature type strain. The diameter of pileus is ca. 46 mm. The total amount of fruit-body production during 4 years(one generation) was 22kg/㎥ log.