The demand for novel strains has been rising in the domestic market to increase the production of sclerotia from Wolfiporia hoelen. To improve strain breeding efficiency, we investigated whether single-nucleotide polymorphisms (SNPs) in the RNA polymerase II subunit (RPB2) gene, which may be linked to the mating type locus, are useful for distinguishing monokaryons from dikaryons in Korean W. hoelen strains. We designed a specific primer set to efficiently amplify a region of RPB2 using PCR with the genomic DNA of 12 cultivated strains and 31 wild strains of W. hoelen collected from Korea. Nucleotide sequences of the PCR-amplified RPB2 genes were determined and analyzed for the presence of SNPs among the 43 W. hoelen strains. Previously reported SNP loci were detected in the RPB2 gene of all W. hoelen strains tested. However, these previously reported SNP loci could not be applied to differentiate monokaryons from dikaryons in approximately one-third of Korean wild strains with homozygous genotypes. Three additional SNPs in the RPB2 gene, which may improve the ability to distinguish monokaryons from dikaryons, were identified by searching through the multiple sequence alignments of the 43 W. hoelen strains. The applicability of these three novel SNPs, together with the previously known SNPs, in the RPB2 gene to W. hoelen strain breeding was verified by examining the hybrid strains and their parental strains.

A new cultivar ‘Sansanhyang’ was bred from monokaryotic strains of Sanbackhyang and SANJO 707ho by mono-mono hybridization method. Cap was convex and brown, it was 70 mm in diameter and 16 mm in thickness. The distribution of scales was whole and the scales were brown and slightly brown. The arrangement of gill was ripple and gill density was dense. Stipe was cream colored and fluff was medium. Sansanhyang had 100day cultivation period and fruiting bodies sporadic occurrence. It was a medium-low temperature strain and the temperature for fruiting body formation was 10-16°C. The ratio of fruiting body production was the 1st flush 89%, the 2nd flush 6% and the 3rd flush 5%. Sansanhyang was different from Sanbackhyang by its convex cap, 70 mm of cap diameter, and 40.5 mm of stipe length. Sanbackhyang had convex cap, 71 mm of cap diameter and 56.9 mm of stipe length.

최근 표고버섯의 맛과 기능성이 알려지면서 동아시아는 물론 전세계적으로 관심이 집중되는 가운데 기후변화와 재배자들의 수요를 충족시킬 수 있는 고품질의 표고버섯 품종개발의 요구도는 날로 높아지는 실정이다. ‘밤빛향’은 산백향과 산조707호을 모본으로 하여 일핵 균주간의 교잡을 통해 새로이 육성된 품종이다. 배양기간 은 100일이며, 발생온도가 11-20°C로 한여름과 한겨울을 제외한 모든 계절에 재배가 가능한 품종이다. 버섯의 형태는 평반구형으로 대는 갓의 중심에 위치하며, 갓의 직 경은 69.6 mm이며, 갓의 두께는 15.2 mm이다. 버섯 갓의 색깔은 진한 갈색으로 일반적인 표고의 갓 색깔보다 짙다 . 버섯의 주름살 측면의 모양은 부정형으로 주름살의 밀도는 보통이지만, 폭이 좁다. 인편의 색깔은 옅은 미(米)색 이며 인편은 갓의 전체에 퍼져 있다. 대의 모양은 기둥형과 깔대기형이 섞인 모양이고, 색깔은 옅은 미(米)색으로 주름살의 색깔과 같다. 대의 표면에는 털이 있으며 털의 색, 대의 털 색, 갓의 인편 모두 옅은 미(米)색으로 존재한다. 버섯은 산발 발생하여 솎아주기가 거의 필요 없다. 모 균주인 산백향과 산조 707호와의 대치배양에서 확연하게 대치선을 형성하였다. 종합적으로 자실체의 형태는 정성적으로 양호한 평가를 얻었고 정량적으로 대조품종 산백 향의 대길이보다 약 9% 줄이는 효과와 갓두께는 16.9% 두꺼운 결과를 얻었다. 목표로 했던 모균주 산조707호의 짧은 대길이의 특성이 유전되었고 산백향의 생산성이 유전된 것으로 판단된다.

노란달걀버섯은(Amanita javanica) 국내에서 산림법으 로 보호받고 있는 식용 가능한 외생균근성 버섯이나, 중요한 산림자원으로서 활용하기 위한 기초적인 특성 연구에 대한 자료는 부족한 실정이다. 이에 본 연구는 국내 채집 야생버섯 자실체로부터 분리한 노란달걀버섯 NIFoS 1267 균주를 이용하여 PDA 배지 상에서 물리적 요인(온도, pH, 광)과 화학적인 요인(염분, 중금속, 농약)에 따른 균사생장 특성을 조사하였다. 최적의 물리적 환경은 온도가 30oC, pH가 5-6, 암조건으로 배양이었을 때 노란달걀 버섯 균주의 균사생장이 가장 좋은 것으로 나타났다. 화학적 요인으로서 염분은 2.0% 농도 조건까지 버섯균주의 균사 생장이 가능하였다. 50 ppm 농도의 중금속 이온 환경에서 비소(As) 이온은 노란달걀버섯 균주의 균사생장에 영향을 주지 않았으나 카드뮴(Cd)와 납(Pb) 이온은 균사 생장이 불가능하게 하였다. 국내 산림에 사용되고 있는 2가지 농약의 경우, Abamectin 첨가 배지에서는 노란달걀 버섯 균주의 균사 생장에 영향이 없었으나 Acetamiprid, Emamectin benzoate, Thiacloprid가 첨가된 배지 환경에서는 균사 생장이 모두 다 저해 되었다. 본 연구 결과는 향후 상업적 생산을 위한 새로운 자원으로서 노란달걀버섯의 인공재배 연구에 도움이 될 것으로 기대된다.

우리나라에서 표고버섯은 소비자의 선호도가 매우 높고, 전체 임산버섯 생산량의 약 97.7%를 차지하고 있는 매우 중요한 단기소득임산물중 하나이다. 이러한 표고버섯은 최근 신품종의 개발이 활발히 이루어지고 있어, 육종가의 권리 보호를 위하여 품종을 구분할 수 있는 분자마커 개발이 요구되고 있다. 본 연구에서는 신품종 ‘산마루2호’를 37개의 표고버섯 품종으로부터 구분할 수 있는 CAPS 마커를 개발하였다. ‘산마루2호’의 유전체에서 Scaffold 2번 1803483에 위치한 단일염기 다형성(SNP)이 확인되었고, 이 SNP를 포함하여 증폭한 DNA는 제한효소 Hhal에 의하여 특이적으로 절단되지 않아 다른 표고버섯 품종들과 구분되었다.

Oak mushroom is one of the most important non-timber forest products at the forest sector in Korea. In recent years, the Korean farmers have suffered from the import increase of saw bed medium from Chinese and the price decrease of the oak mushroom. In order to overcome this phase, it is important to develop good varieties that can compete with Chinese varieties. National Institute of Forest Science is studying to develop excellent varieties through the Golded Seed Project. We report some of the results as part of that. The strains of oak mushroom were made through mono-mono cross method using domestic distribution varieties. Sawdust cultivation was carried out on 100 - day cultivation and 3 - stage mushroom yield using 2 kg hexahedron culture medium. Of these, 60 strains were used for sawdust cultivation, and 3431 strain (23%) was selected when the yield was 20% or more of the weight of the medium. Four strains (3396, 3414, 3419, 3430) were selected when the mushroom yield was 15% to 20% of the weight of the medium. 3404 strain with excellent mushroom shape was selected, though the yield was low (10%). Generally, mushroom yield was the highest in the first stage, and the number of mushrooms in the second stage was decreased rapidly. Also, 10 strains did not produce mushroom in this study. The 3431 strain occurred mushroom production with 87% yields in the first stage and did not occur in the third stage. The 3404 strain with excellent mushroom shape occurred in primary and tertiary. The average weight of mushrooms was 13 g and 54g, respectively. The strains (3431, 3404) will be used for the selection of varieties through cultivation test in the future.

Suillus granulatus is an ectomycorrhizal fungus that is associated with pine roots. S. granulatus is edible mushroom and known as the weeping bolete. S. granulatus is similar to S. luteus, but S. granulatus has a ringless stalk. We investigated in the growth characteristics of the 4 strains of S. granulatus with growth medium, temperature, and cellulase enzyme activities. NIFoS 1211 and 2701 showed the highest growth on PDA and NIFoS 1997 produced abundant mycelia on MMN. NIFoS 2615 was grown well on SDA. In spite of same species, the optimal media were different according to habitat of fruit body. The optimal temperature for mycelial growth of all strains was 25oC and NIFoS 2615 was also grown well at 30oC. All of strains on MMN liquid medium were typically grown at pH ranges from 5.8 to 6.2. The dry weights of S. granulatus strains were measured according to different inorganic nitrogen sources. All strains showed the highest dry weight on MMN and showed little difference on none contained nitrogen source, ammonium and nitrate media. We measured cellulase activity on carboxymethylcellulose (CMC) agar plates, and determined clear zone according to pHs. The 4 strains of S. granulatus showed the positive reactions in cellulase activity. All strains formed the height clear zone at pH 6.

For centuries, Lentinula edodes called “Pyogo” in Korea has been cultivated as a high-grade edible mushroom in Asia. This is known as medicinal mushroom for cold prevention in oriental medicine. In the world market, this mushroom of Lentinula edodes is introduced as representative edible and medicinal mushroom in Asia. As consumer interest increases, Asian Lentinula edodes market is also growing. There is a record that Korea has already been used as a gourmet food on the royal meal table for the king in the 15th century Joseon Dynasty. In Korea's famous oriental book, it is cooked with many traditional foods because of the pharmaceutical efficacy of this mushroom. For long time ago, difference in cultivation as well as in mushroom growth of Lentinula edodes has studied in Korea. Recent advances in cultivation of selected high quality mushroom studied from substrate optimization using log and sawdust of oak tree. Cultivation of Lentinula edodes strain research controlled the production of large quantity of various substrate conditions. This mushroom mycelia and fruit-body were cultivated in log of oak tree and sawdust substrate cultivation in types of bioreactors. According to compared cultivation method, it presented the optimistic condition for the amount production of various Lentinula edodes strain in Korea.

Lentinula edodes is a very important edible mushroom and is widely cultivated in Asian countries using a large number of varieties. National Institute of Forest Science (NIFoS) conducts Golden Seed Project to develop new shiitake varieties and we examined cultural characteristics of seven candidate strains compared to three existing cultivars. As a result, optimal growth temperature and mycelial growth on potato dextrose agar (PDA) for seven days at the optimal temperature were 27°C, 55 mm in Sanbaekhyang, 27°C, 50 mm in Sanjo701ho, and 25°C, 49 mm in Sanjo707ho, respectively. Five of the candidate strains except NIFoS 3309 and 3310 showed the highest growth at 25°C. Mycelial growth of NIFoS 2889 and 2905 showed the fastest (60 mm) and the slowest (32 mm), respectively. Six strains showed bulging on surface of media in sawdust bag cultivation for 30 days. In particular, the cultiver, Sanjo701ho had heavy bulging. Four strains (NIFoS 624, 971, 3310, and 3363) did not show bulging and NIFoS 971 grew slower than the others. Keywords : Browning, Characteristics, Lentinula edodes, Mycelial growth, Tunicate

In this study, we investigated the amino acids content of shiitake mushrooms grown in eight different media and under three different temperature conditions. The sawdust media were supplemented with 4 kinds of nutrients - rice bran, barley, bean curd refuse, and red ginseng refuse in an 8:2 ratio with or without 0.5% calcium chloride, and the fruiting temperature was set to low (13–15oC), medium (18–20oC), or high temperatures (24–26oC). Seventeen amino acids were detected, and the content varied depending on the compositions of the media. The highest total amino acid content (209.7 mg/g) was measured in mushrooms from the media with red ginseng refuse, and the glutamic acid content was the highest (51.3 mg/g) in mushrooms from the media with barley. In terms of fruiting temperature, the amino acid content was the highest (38.2%) at medium temperature followed by the content at low (32.5%) and high temperature (29.3%). The amino acid composition of shiitake mushrooms was similar between the medium and low temperature conditions.

Sawdust bag cultivation was performed to understand the effect of different sawdust substrate (Quercus mongolica, Q. acutissima, Q. serrata, Q. aliena, and Q. variabilis) on the eight variety of shiitake (Poongnyunko, SANJO 701, Mori 436ho, Sanlim 10ho, Chilha 6ho, Yoogiro, Baekwhahyang, Charmaram). After inoculation, sawdust substrate block (2 kg) was cultivated at 22°C for 120 days. During the production period, the temperature was adjusted to 18°C and the relative humidity at 90%. The yield was calculated based on the fruit bodies from the first, second and third flush. As results, Poongnyunko yielded 455 g on the substrate, Q. serrata, 378 g on Q. aliena, and 333 g on Q. variabilis. Chilha 6ho yielded 304 g on Q. mongolica and Charmaram yielded 245 g on Q. acutissima. The average weight of mushrooms from Charmaram was 40-45 g on all five substrates and Q. variabilis was the best among them. In terms of phenotypic characteristics, Chilha 6ho was generally better than the others. Charmaram, Poongnyunko, Sanlim 10ho also had good characteristics. Mori 436ho, Yoogiro, Baekwhahyang developed for log cultivation showed lower yield compared to the varieties for sawdust bag cultivation. Keywords : Lentinula edodes (Shiitake), Querqus spp., Saw-dust bag culture, Yield

New shiitake (Lentinula edodes) strains “Dasanhyang”, “Chunjang-1ho” and “Poongnyunko” were breeded for bed-log cultivation. Fruit-body production of “Dasanhyang” was most at summer and autumn. The total amount of fruit-body production during 4 years(one generation) was 31kg/㎥ log. The fruit-body is hemispherical shape, brown colored and diameter of pileus is ca. 54 mm. Optimal temperature of fruit-body formation was 14~2 2℃, and the fruiting is concentrated. Fruit-body production of “Chunjang-1ho” was most at spring and autumn. Optimal temperature of fruit-body formation was 12~22℃ and range of fruiting temperature is broad. The diameter of pileus is ca. 46 mm. The fruiting is concentrated, and the total amount of fruit-body production during 4 years(one generation) was ca. 18kg/㎥ log. Fruit-body production of “Poongnyunko” was most at summer and autumn. Optimal temperature fruit-body formation was 17~24℃. It is high-temperature type strain. The diameter of pileus is ca. 46 mm. The total amount of fruit-body production during 4 years(one generation) was 22kg/㎥ log.

For diversification of materials for sawdust cultivation of Lentinula edodes, this study was carried out to examine adding effects of corncob and cottonseed meal on sawdust cultivation. All treatments of corncob except 100% corncob have produced fruiting-bodies. Mushroom productivity of the fungus was decreased from 174g to 45g per 2kg sawdust-based substrate in proportion to increasing corncob contents in sawdust cultivation. Meanwhile, in treatments including less than 20% cottonseed meal, the mycelial growth of the fungus was similar to control. But, the other treatments were not suitable for the mycelial growth of fungus on sawdust-based substrate. All treatments of cottonseed meal did not produce the fruiting-body of the fungus.