The sensitivities of PrP Sc detection methods, western blotting (WB), immunohistochemistry (IHC) and protein mis-folding cyclic amplification (PMCA) techniques were compared from brains, spleens and blood of mice challenged with PrP Sc of murine-adapted BSE strain 301C. PrP Sc was detected in the spleen from 30 dpi by IHC and at 60 dpi by WB. At 30 dpi, disease-specific signals of PrP Sc was observed in only two follicles of a single spleen. PrP Sc was detected in spleen at 10 dpi with PMCA after 5 rounds of amplification. Clinical signs were obviously shown from 240 dpi, and coincided with first detection of PrP Sc in brains by WB, IHC and PMCA after one round amplification. In addition, PrP Sc was also detected in blood at 60, 180 and 240 dpi with PMCA after 5 rounds of amplification. The FDC-M1 epitope, which appears in immature FDCs, and PrP Sc were detected in follicles first at 30 dpi, whilst the FDC-M2 epitope of mature FDCs was detected at 60 dpi. More FDC-M2 epitope and PrP Sc were detected in follicles as disease progressed. The CD21/35 epitope is expressed on both FDCs and germinal center B cells. The pattern of CD21/35 expressing cells was similar to but less dominant than that of FDCs.

목적: PRP(범안저레이저광응고술) 시행 전에 측정한 망막정맥충만시간의 절대치 뿐만 아니라, PRP 시행 전과 후에 그 변화량을 평가함으로써, 그 변화량과 신생혈관 진행과의 연관성을 규명하고자 하였다. 방법: 증식당뇨 망막병증으로 본원에서 PRP를 시행한 환자 중 PRP 전과 후에 형광안저촬영을 시행하였던 환자 32안을 주사형 레이저검안경을 이용한 형광안저촬영 시행 후 2주 내에 PRP를 시작하였고 PRP 시행 3~5개월 후에 다시 형광안저촬영을 시행하였다. 결과: 망막정맥충만 시간의 PRP 전 후의 변화는 안정화된 군에서는 -0.99±1.60초 감소하였고 불안정화된 군에서는 0.30±1.69초 증가하였으며 독립 T-검정상 망막정맥충만시간의 변화는 통계적으로 유의하였다(P