Bovine viral diarrhea virus (BVDV) is a major pathogen that may be one of the main reasons for economic losses in the livestock industry. BVDV is a well-characterized member of Flaviviridae family with plus-stranded RNA viruses. Non-structural NS5B protein is RNA-dependent RNA polymerase, which is responsible for viral RNA synthesis and genome replication of BVDV. Therefore, the NS5B polymerase is a key target for the discovery of anti-BVDV drugs. A number of small-molecule inhibitors against the NS5B polymerase have been reported in literature of which we collected series molecules having various scaffold with their biological data determined by evident experimental conditions, methods and procedures. Then, we constructed database of 655 small-molecule NS5B inhibitors having definitive activity values, structural parameters, and physicochemical properties (such as molecular hydrophobicity, hydrophilicity, polarity, Hbond donors and H-bond acceptors) associated with their absorption and permeability through a cheminformatics approach. The database was opened to provide insight for allosteric NS5B inhibitors of BVDV with an accessible platform on the web (http://nabic.rda.go.kr/chemical genomic database/BVDV RNA dependent RNA polymerase inhibitors). This molecular information in the database would be useful in attempting to identify features and decision factors that enhance anti-BVDV activity or increase selectivity of the allosteric inhibitor. These anti-BVDV molecules could also be screening for the purpose of exploiting potent NS5B inhibitors in the same family (e.g., HCV, CSFV, YFV, WNV, and DENV).

Black queen cell virus (BQCV), one of the most prevalent viruse, causes the death of queen larvae and pupae. The RNA-dependent RNA polymerases (RdRPs) are central components in the life cycle of RNA viruses that catalyzes the replication of RNA from an RNA template without DNA stage. Inhibition of RdRP gene is importantly significant for application of monoclonal antibody generation as a diagnosis tool for identifying BQCV infection in honey bee..In this study, the presence of BQCV in honey bee samples was confirmed by PCR using BQCV F/R primer set to multiply of 700 bp DNA fragment. For ampification of BQCV Rdrp gene, a primer set attached BamHI/SalI restriction site was designed based on the best homogenization between BQCV RdRP sequences in NCBI, a PCR product containing BQCV RdRP gene with 1576 bp in length was amplified. Furthermore, BQCV RdRP gene will be cloned into pBlueXcm vector for future researches.

Sacbood virus (SBV) is an infectious disease, resulting in failure to pupate and death and kSBV is a disease caused perish Apis cerana of 75% in South Korea. RNA dependent RNA Polymerase(RdRP) is one of polyprotein of viral genome and an enzyme that catalyzes the replication of RNA from an RNA templates and an essential protein encoded in the genomes of all RNA containing viruses with no DNA stages. In this study, recombinant construct with RdRP partial region of kSBV was used for sequence analysis to clarify about Korean SBV. As a result it could be determined that the virus develops by infection of Korean Apis cerana called kSBV. Also, we named Apis cerana-kSBV-region to the name of the unique region of gene that kSBV has. In comparison of the RdRP region of bee RNA virus on nucleotide sequence, its sequence from same species have less variability as well as each virus species has a certainty of RdRp region. It indicated that mutations of RdRP region of each virus species is able to be a useful indicator of honeybee virus detection.

Deformed wing virus(DWV) is one of infectious disease of honey bee that is caused to wings of immature or mutation and death at last. In this study 4 kinds of polyprotein of DWV are compared and then selected 2 kinds of polyprotein, RNA-dependent RNA polymerase(RdRP) and peptidase C3G, which has relatively higher homology than others. Analysis of both RdRP region and peptidase C3G of DWV deposited in Genbank of NCBI showed 99~100% and 95~97% of homology on genomic level, respectively whereas analysis of CRPV-capsid and RNA helicase showed 86~98% and 89~98% of homology, repectively. According to the result of gene analysis, two kinds of polyprotein are constructed and analyzed the homology, resulting in RdRP and Peptidase -C3G showed about 96% and 97% of homology, respectively. It indicated that both region is able to use for generation of specific antibody for the diagnosis of Deformed Wing Virus (DWV).