This work was performed to confirm the molecular discrimination through the nrITS1 sequences among 3 taxa of Scirpus L. sensu lato (s.l.) species. S. planiculmis represented only 2 base sequence variations with S. maritimus in spite that they showed different morphological features. The nucleotide sequences of the ITS1 region from S. planiculmis were shown to have 99.1% homology with S. maritimus and 60.4% homology with S. triqueter. Although the morphology of S. planiculmis is similar with S. triqueter, molecular basis of the size and sequences on ITS1 region were shown to have distinctive differences. For divergency investigation on same sites and metapopulation, sequencing was conducted on ITS1 region with partial 5.8S and 18S regions. All plants of each species collected at the same site had identical band size pattern and sequences. Intraspecific molecular divergency was not identified in spite that these species live in different wetland sites. The ITS1 sequences described here provided a powerful genetic tool for phylogenetic studies which was difficult by morphological identification as high rate of morphological plasticity

Five known anthraquinones, physcion (1), I-O-methylemodin (2), emodin (3), physcion-8-O-β,-D-glucopyranoside (5), emodin-8-O-β,-D-glucopyranoside (6) and two known stilbenes, trans-resveratrol (4), trans-resveratrol-3-O-β,-D-glucopyranoside (7) were isolated from MeOH extract of Reynoutria sachalinensis (Polygonaceae). All structures were unambiguously established by 1D and 2D NMR and MS data and the compounds were evaluated for their cytotoxicity against L1210, HL-60, BI6F10 tumor cell lines in MTT assay. Among the compounds, trans-resveratrol (4) exhibited significant cytotoxic activity with IC50 values of 9.2, 6.7 and 9.8 μg/ml, against the test cell lines respectively, but compounds 1-3 exhibited the moderate cytotoxic activity.

갯방풍(防風)의 품질(品質)은 홍색(紅色)의 착색과정(着色過程)에 따라 크게 좌우(左右)되기 때문에 보다 확실(確實)한 착색기술(着色技術)의 확립(確立)이 요망(要望)된다. 따라서 본 실험(實驗)은 갯방풍(防風)의 Anthocyanin 생성(生成)에 미치는 환경요인(環境要因)을 구명(究明)하고자 수행(遂行)하였던 바 얻어진 결과(結果)는 다음과 같다. 1. 온도처리(溫度處理)에 따른 갯방풍(防風)의 발아율(發芽率)은 20/15℃ 에서 가장 높았고, 30/25℃에서는 거의 발아(發芽)가 되지 않았다. 또한 지상부(地上部) 생체중(生體重)은 25/20℃에서 가장 양호(良好)했다. Anthocyanin 생성(生成)은 15/10℃의 온도(溫度)에서 가장 높았고, 30/25℃의 고온(高溫)에서 억제(抑制) 되었다. 2. 각(各) 온도조건(溫度條件)에서 광처리구(光處理區) 간(間)의 지상부(地上部) 생체중(生體重)에는 큰차가 없었다. 또한 광(光)은 Anthocyanin 생성(生成)을 촉진(促進)하는 것으로 인정(認定)되었으며, 특히 광(光)의 그 촉진작용(促進作用)은 저온(低溫)에서 더욱 효과적(效果的)이었다. 3. 자외선처리(紫外線處理)에 따른 지상부생육(地上部生育)은 전반적(全般的)으로 큰차는 없었지만, 25/20℃에서는 자외선(紫外線) 투과구(透過區)가 자외선(紫外線) 차단구(遮斷區)에 비해 양호(良好)한 경향(傾向)을 보였다. Anthocyanin생성(生成)은 자외선투과구(자외선투과구)가 자외선차단구(紫外線遮斷區) 보다 많았던 점으로 보아 Anthocyanin생성(生成)에 대한 자외선(紫外線)의 효과(效果)가 인정(認定)되었다.