We investigated the biochemical composition and functional properties of garlic sprouted via hydroponic cultivation. Sprout length and sprouted garlic weight increased 6.9-fold (97.94±22.47 mm) and 1.4-fold (9.11±1.32 g), respectively, by day 20. Proximate compositions, including protein, fat, ash, and carbohydrates, were analyzed to determine nutrient changes. Notably, protein content increased from 17.73% to 20.21%. Minerals including phosphorus, potassium, magnesium, and iron varied significantly, supporting metabolic processes. Total free amino acids, particularly arginine and glutamine, increased from 5,301.21 mg% to 6,962.80 mg%, suggesting potential improvements in nutritional value and flavor. Protease activity reached its maximum at 21.26±2.47 mU/mL on day 20, promoting protein hydrolysis, and amylase activity steadily increased, reaching 299.82±0.85 mU/mL, providing energy through starch breakdown. Vitamin C content reached a maximum on day 8 (307.25 mg/kg, suggesting that vitamin C is produced during early sprouting. Phenolic compounds, which are critical for antioxidant activity, reached their highest concentration (42.15 mg/100 g) by day 12, correlating with increases in DPPH and ABTS radical-scavenging activities. These findings indicate the potential of sprouted garlic as a functional ingredient. Future research should optimize hydroponic cultivation conditions to improve bioactive compound content and antioxidant properties, contributing to the development of high-value functional foods.