Mitochondria is energy generating organelle. It synthesizes ATP, which is the essential energy source of many cellular processes. During producing energy, some redox centres leak electrons to oxygen and it is contributory to the reactive oxygen species. Besides, mitochondria have significant functions in metabolism, calcium homeostasis, and fatty acid oxidation.
Also mitochondria has importance to the breakdown of the ovarian follicles and could be factor determining oocyte of quality adversely. Increasing evidence shows that the number of mitochondria affect oocyte of developmental competence and maturation detrimentally during aging. Oocyte is the mitochondria-rich cell and enable the organelle to have competence for fertilization and early embryonic development. Occurrence of blastomere depends on distribution change of mitochondria which present in the egg.
Lonicera caerulea treatment inhibited ovarian mitochondrial oxidative damage by suppressing mitochondrial reactive oxygen species (mROS) generation, decreasing apoptosis, controlling disintegration of mitochondrial membrane potential and conserving respiratory chain complex activities.
The purpose of this study is to identify if mouse accepting treatment with L. caerulea could counter age-induced sterility and ovarian mitochondrial OS in a model organism of ovarian ageing.
The citrus flavonoid hesperetin has various pharmacological actions, including antioxidant, anti-inflammatory, and anticancer activities. The purpose of this study is to confirm whether the treatment of hesperetin can protect the oocyte from in vitro aging. Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 1, 10, 100, and 250 μM hesperetin (aging, H-1, H-10, H-100 and H-250, respectively). This study investigated the effect of different concentration of hesperetin on maturation, and reactive oxygen species (ROS) level, apoptosis index, and the developmental capacity of aging porcine oocytes. In the results, the percentage of cleaved oocytes that reached to the blastocyst stage of H-100 group (37.9 ± 1.1%) was similar to control (38.1 ± 0.8%), and also significantly higher than other aging groups (23.2 ± 0.8%; H-1, 19.7 ± 1.3%; H-10, 26.7 ± 0.6%; and H-250, 18.4 ± 1.6%.)(p<0.05). The H-100 group was significantly decreased ROS activity, and increased the level of glutathione (GSH) and expression of the antioxidant genes (PRDX5, NFE2L, SOD1 and SOD2) compared to the aging group. The H-100 groups prevented aberrant spindle organization and chromosomal misalignment, blocked the decrease in the level of phosphorylated-p44/42 mitogen-activated protein kinase (MAPK), and increased the mRNA expression of cytoplasmic maturation factor genes (GDF9, CCNB1, BMP15 and MOS). Also, it was confirmed that the H-100 group expressed higher level of estrogen receptor than the aging group. Therefore, this result indicated that hesperetin is an effective agent to protect from the oxidative stress during in vitro aging of porcine oocytes.
This paper investigates tensile characteristics of the stress aging heat-treated SM45C steel which are aging temperature at 250℃, 300℃, aging time at 1, 3 hours, and applied load at 300, 400N conditions by using acoustic emission. Most suitable aging condition was aging temperature 300℃, aging time 1 hour, and aging applied load 300N. And increased yield load 28.3% than non-treatment specimen in this condition. AE energy in elastic limit increased about 16.7 times than non-treatment specimen. When aging time is 3 hours, yield load decreased than other conditions that possibility is high to have itself defect on inside the specimen or coarse grain size precipitation is different in happened over-aging phenomenon. Especially, in case of 300℃, 3 hours and 400N condition appeared AE energy in elastic limit fairly high about 30 times than non-treatment specimen. This is considered by emit a lot of energies when material causes plastic deformation because the ductility increases on specimen by over-aging phenomenon.
유기도막의 방식성능은 도막의 수지성분과 안료의 화학적 특성에 의존한다 전자는 부식인자의 침투를 차단 및 지연시키는 역할을 하며, 후자는 침투된 부식인자들에 의해 일어나는 부식반응을 억제하는 기능을 갖고 있다. 또한 도막 자체의 영향 외에, 외부 환경에 의해서 다르게 나타난다. 본 연구에서는 교류 임피던스 법을 이용하여 유속과 그에 따른 유동 전단응력에 의한 도막의 열화거동을 조사하였다. 실험기 사용된 도막의 두께는 70μm에서 140μm까지 변화시켰다. 두꺼운 도막(140μm) 에서는 유속이 증가함에 따라 물 흡수량의 감소와 높은 임피던스 특성을 나타내었다. 그러나 얇은 도막(90μm) 에서는 유속이 증가함에 따라 도막의 파괴가 진행되고 있음을 확인할 수 있었다. 도막에 가해지는 전단응력이 증가할수록, 즉 선박의 운항속도가 증대될수록 도막의 열화에 의하여 방식성능이 떨어짐을 확인할 수 있었다.
7050 Al합금의 응력부식저항성에 미치는 2단 시효처리의 영향을 미세조직관찰, 전기전도도시험 및 SCF값을 평가함으로써 조사하였다. 최대 경도 이상 과시효에 의해 주 강화상인 η'상이 η상으로 변태되었고, 입내 및 입계에 존재하는 η상의 크기와 간격이 커졌으며 그 결과 응력부식저항성이 증대되었다. 2차 시효시간의 증가에 따라 전기전도도값은 증가되었으나 항복강도의 감소로 인하여 SCF값은 감소되었는데, 이러한 결과는 응력부식저항성의 증가를 의미한다. AMS 4107규격을 기준으로 하였을 때 7050 Al합금 단조재의 적정시효조건은 1차시효가 120˚C에서 6시간, 2차시효는 175˚C에서 12시간이었다.