Sex hormones including progesterons, androgens, and estrogens are influential in differentiation of ovarian tissues and competence of fertility. These steroid hormones derived from cholesterol are required for cumulus-oocyte complexes(COCs) during oocyte maturation. COCs is a total functional and active entity playing a central role in oocyte. Lipid metabolism in the mammalian COCs is controlled by environmental factors. The intracellular cholesterol contents go through remarkable changes. It plays an important part of oocyte developmental competence. However, heat stress affects steroid hormone by decreasing progesterone, estrogen concentrations, and resumption of meiosis in COCs maturation. Reduction of the hormone and meiotic resumption might lead to the decline of ovarian function, follicle maturation, and subsequent embryogenesis. In the same vein, heat stress also influence on germinal vesicle breakdown, lipolytic variations, and loss of the nuclear envelope in the course of maturation of oocytes. In summary, we examined the effects of thermal stress on oocyte maturation through steroid hormone contents of change identifying the molecular mechanisms of lipids metabolism. It may have the solution to further the therapy methods for disorders regarding sterility.

The purpose of this study was to investigate the effect of rapid weight reduction for short duration on serum enzyme and stress hormone in high school wrestlers. Subjects were composed of 7 student wrestlers whose wrestling careers were 3years over. Subjects were reduced 5% of their body weight by exercise, reducing food and sauna method for three days. On the basis of the results analyzed in this study and consideration of many pre studies related it, the result could be drawn as follows. First, Data analysis of 5% weight reduction on CPK, post level revealed more significant increase than pre level(p<.05). Second, Data analysis of 5% weight reduction on LDH, post level revealed more significant increase than pre level(p<.05). Data analysis of 5% weight reduction on Epinephrine, post level revealed more significant decrease than pre level(p<.05). Data analysis of 5% weight reduction on Norepinephrine, post level revealed more significant increase than pre level(p<.05). In conclusion, rapid weight reduction gives an effects on decreasing wrestlers' physical fitness suddenly during the match because of changing rapidly of serum enzyme and stress hormone in the body. I hope that more studies will be followed on serum enzyme and stress hormone in rapid weight reduction of weight class competition players in the future.

The TIFY family is composed of a plant-specific group of genes with diversity of functions. This family represents four subfamily of proteins viz. ZML, TIFY, PPD and JASMONATE ZIM-domain (JAZ) proteins. TIFY proteins especially, JAZ proteins have been reported to perform different biological processes, such as developmental and stresses and hormone responses in Arabidopsis and rice. However, there is no information about this family genes in Brassicaceae. This study identifies 36 TIFY genes in Brassica rapa, an economically important crop species from this family. An extensive in silico analysis through phylogenetic grouping, protein motif organization and intron-exon distribution also confirmed 4 subfamilies of BrTIFY proteins. Out of 35 BrTIFY genes, we identified 21 under JAZ subfamily besides 7 TIFY, 6 ZML and 2 PPD. An extensive expression profiling of 21 BrTIFY JAZs both in tissues and organs of B. rapa revealed differential expression patterns. Almost all the BrTIFY JAZs predominantly expressed in leaves and flower buds. Besides, in a flower stage specific expression analysis we observed 14 BrTIFY JAZs with constitutive expression patterns. This indicates BrTIFY proteins have a strong involvement in the development of B. rapa flowers. Our protein interaction study also reveals the strong association of these proteins with the fertility and defense processes of B. rapa. To elucidate the stress responsiveness of BrTIFY genes, we analyzed the low temperature-treated whole-genome microarray data set and found almost all the BrTIFY JAZs were having variable transcript abundance in two contrasting inbred lines of B. rapa. Subsequently, all 21 BrTIFY JAZs were validated in response to cold stress in the same two lines via qPCR, where 9 genes were found to show up- regulation. And, a high and differential qPCR expression pattern of all the BrTIFY JAZs was also recorded against JA. Additionally, BrTIFY JAZs were tested against salt, drought, Fusarium, ABA and SA treatments and a considerable number of genes were found to be induced. The extensive annotation and transcriptome profiling reported in this study will be useful for understanding the involvement of TIFY genes in stress resistance and different developmental functions, which ultimately provides the basis for functional characterization and exploitation of the candidate genes for genetic engineering of B. rapa.

본 연구의 목적은 장기간 저항운동 강도별 스트레스를 지칭하는 호르몬의 분비 차이를 제시하는 것이며, 타 연구 논의의 차별화로 배제된 심리적 관련 변인을 기술하였다. 연구대상은 C 대학교 남자대학생 17명으로 최대하 및 최대운동 강도별로 각각 6주간의 저항운동 실시 후 네 시점별 성장호르몬과 테스토스테론 및 코르티솔의 차이 결과, 남성호르몬을 제외한 성장 및 코르티솔 변화는 안정시에 비해 운동직후 유의하게 증가하였으며, 6주 전후 변화는 코르티솔만이 네 시점별 유의한 증가가 있었다. 분비 차이의 의미로, 성장호르몬 증가는 운동 강도별 모두 유의하게 증가하였으며, 남성호르몬은 유의한 변화가 없었으며, 최대강도 운동의 경우 코르티솔은 6주 후 평소 안정 시에도 증가되어 있었다. 장기간 운동처치의 생리적인 결과 해석에 심리적 변화를 고려하여야 하며 부정적인 스트레스로 변환되지 않도록 심리적인 여러 변인 연구가 병행되어야 하겠다.