In order to obtain the fundamental data about the behavior of sharks by underwater audible sound, this experiment was carried out to investigate the auditory characteristics of tiger shark Scyliorhinus torazame which was caught in the coast of Jeju Island by heart rate conditioning method using pure tones coupled with a delayed electric shock. The audible range of tiger shark extended from 80Hz to 300Hz with a peak sensitivity at 80Hz including less sensitivity at 300Hz. The mean auditory thresholds of tiger shark at the frequencies of 80Hz, 100Hz, 200Hz and 300Hz were 90dB, 103dB, 94dB and 115dB, respectively. The positive response of tiger shark was not evident after the sound projection of over 300Hz. At the results, the sensitive frequency range of tiger shark is narrower than that of fish that has swim bladder. In addition, it is assumed that the most sensitive frequency in auditory thresholds of Chondrichthyes is lower than that of Osteichthyes. Critical ratios of tiger shark measured in the presence of masking noise in the spectrum level range of about 60-70dB (0dB re 1μPa/Hz) increased from minimum 27dB to maximum 39dB at test frequencies of 80-200Hz. The noise spectrum level at the start of masking was distributed at the range of about 65dB within 80-200Hz.