In this study, the effect of different substrates of agricultural by-products on the mycelial growth rate and density of Trametes versicolor (Turkeytail mushroom) was analyzed. We found that pepper stem and rice bran with a mixing ratio of 9:1(v/v) produced the best mycelial growth of 101 mm in 10 days, while a mixing ratio of 8:2 resulted in mycelial growth of 83 mm in 10 days. The control group treated with a 9:1 mixing ratio of oak sawdust and rice bran (v/v) produced mycelial growth of 74 mm in 10 days. The following results are in the order of beanstalk, sesame stem, and perilla stem. After the harvest of the mushrooms, the mycelial growth rate and the density of T. versicolor in each substrate were as follows the group with waste substrate of Pleurotus eryngii and rice bran with a mixing ratio of 9:1(v/v) produced the best result of 76 mm in days, while a mixing ratio of 8:2 produced of 61 mm in 10 days. The control group with a 9:1 ratio of oak sawdust and rice bran produced mycelia of 74 mm in 10 days, while a mixing ratio of 8:2 resulted in mycelia of 59 mm in10 days.

구름버섯(Trametes versicolor)은 phenolic compound인 CV와 MG를 효과적으로 탈색할 수 있었으며 고체와 액체배양 상태 모두에서 CV보다 MG를 더 효과적으로 탈색시켰다. 구름버섯에 의한 두 색소의 탈색 과정에서 phenolic compounds를 분해하는 것으로 알려진 세 가지 효소 중 laccase의 활성이 가장 높았다. MnP 역시 적은 수치지만 활성을 나타냈으며 LiP의 활성은 나타나지 않았다. 따라서 구름버섯에 의한 합성염료의 분해과정에서 laccase가 주로 사용되고 MnP는 탈색과정에서 보조적인 작용을 하는 것으로 추정된다. 그러나 CV의 경우 MnP가 활발하게 염료분해에 관여하는 것으로 판단된다. 또한 MG가 대부분 탈색되었을 때의 laccase 활성(0.16 U/mg)이 CV가 대부분 탈색되었을 때의 활성(0.23 U/mg)보다 현저하게 낮은 것으로 보아 구름버섯이 CV를 탈색시키는데 더 높은 활성의 laccase가 필요로 하는 것이 밝혀졌다. 본 실험에서 한국산 구름버섯 종의 CV와 MG 탈색능력이 확인되었으며 앞으로 한국산 구름버섯을 이용한 triphenyl methane계에 속하는 합성염료의 분해에 관한 친환경적 처리기술 개발에 도움이 될 것으로 기대된다.

Trametes versicolor showed the ability of degrading synthetic dyes such as congo red (CR) and methylene blue (MB) in solid and liquid culture conditions. The T. versicolor strains isolated in Korea degraded MB more efficiently than CR, differently most of other white mushrooms known to have difficulties in degrading MB than other dyes. Thus the Koren strains of T. versicolor showed the commercial potential to be used for cleaning dye-contaminated region without any patent-related problem. The main enzyme responsible for dye deradation was laccase. The manganese peroxidase (MnP) was also detected and supposed to be involved in the degradation process of synthetic dyes. However, no lignin peroxidase (LiP) was detected from degradation process, indicating LiP is not the enzyme T. versicolor use to degrade CR and MB.