The purpose of this study was to investigate radiation dose sensitivity due to displacement of human extremities in the water bolus box on radiation therapy. Water bolus box and human thigh with femur bone were constructed in computerized radiation therapy planning system to verify the absorbed dose. Two 6MV X-ray beams were irradiated bilaterally into water bolus box and then radiation dose were calculated each situation at displacement of middle axis of thigh from the center in water bolus box to right and left direction. Absorbed dose of thigh and femur bone increased by the distance of displacement. The maximum dose of thigh even increased 20% over than prescribed dose. This is in contrast to conventional concept of dose distribution in water bolus box. Based on this result, displacement of body site in the water bolus box have to be averted during radiation therapy.