Paecilomyces tenuipes (P. tenuipes) is a fungus cultivated artificially by South Korean researchers, utilizing rice bran as its substrate. The increased demand for this fungus has not been met with successful cultivation methods for fruiting body production in natural environments. Therefore, we tested the effect on the growth of P. tenuipes using a Solid media based on pests. In this results, the Solid media based on M.alternatus was effective in increasing the growth of P. tenuipes and the content of cordycepin. Moreover, we confirmed the conditions for manufacturing a Solid media based on M.alternatus for P. tenuipes growth. We suggested that the growth-promoting compounds offers valuable insights for optimizing fungal cultivation conditions, thereby enhancing productivity and contributing to a broader understanding of fungal physiology in varying nutritional environments.

To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Aristolochia tagala Champ. was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by column chromatography and obtained forty three subfractions. The forty three fractions were searched the anti-fungal activities by bioassay. The most active No. 26 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, 2,4-di-tetra-butyl-phenol, 2-mono-palmitin, 1-mono-stearin were profiled as maine compounds in No. 26 subfraction. Bioassay using commercial 1-mono-stearin to test for the anti-microbial activity conformed the antimicrobial active compound. In conclusion, 1-mono-stearin identified from Aristolochia tagala Champ. was antimicrobial chemical against Sclerotinia sclerotiorum.

To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Usnea longissima was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by silica gel-column chromatography and obtained into nine group subfractions. The nine group fractions were searched the antifungal activities by bioassay. The most active No. 3 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to database of Wiley library. As a result, Usnic acid was identified as main compounds. In conclusion, Usnic acid isolated from Usnea longissima was antimicrobial chemical against Sclerotinia sclerotiorum as a pathogen of sclerotium disease.

본 연구는 남부지역에서 주요 콩 품종의 파종기 이동이 isoflavone 등 생리활성물질에 미치는 영향을 구명하고자 전남 나주(위도 35° 04'N, 경도 126° 54'E)에서 2008년부터 2010년까지 3년간 수행되었다. 여름형콩 품종인 새올콩과 다원콩, 가을형콩 품종인 태광콩, 풍산나물콩 및 청자3호를 이용하여 파종기를 5월 15일, 5월 30일, 6월 15일, 6월 30일, 7월 15일에 실시하였으며, 주요 결과를 요약하면 다음과 같다. 1. Daidzein 함량은 모든 시험품종에서 genistein과 total isoflavone 함량은 청자3호를 제외한 시험품종에서 파종이 늦어질수록 증가하는 경향이었다. 반면에 glycitein 함량은 다원콩을 제외한 시험품종에서 파종기간 유의차가 없었다. 2. Cyanidin-3-glucoside(C3G) 함량은 다원콩과 청자3호, delphinidin-3-glucoside(D3G)와 total anthocyanin 함량은 다원콩에서만 파종이 늦어질수록 유의하게 증가하였다. 그러나 pelargonidin-3-glucoside(Pg3G)와 petunidin-3-glucoside(Pt3G) 함량은 파종기간 유의차가 없었다. 3. 청자3호의 lutein 함량은 파종기에 따라 2.7～3.0 μg/g로 유의차가 없었고, chlorophyll 함량은 파종이 늦어짐에 따라 증가하여 6월 30일과 7월 15일 파종에서 유의하게 많았다. 4. 조지방 함량은 태광콩과 청자3호는 조기에 파종할 때 많았고 기타 시험품종은 파종기간 유의차가 없었다. Palmitic acid 조성비는 대부분 시험품종에서 파종기의 영향을 받지 않았다. Stearic acid 조성비는 새올콩, 다원콩, 청자3호에서 파종이 늦을수록 증가하였다. Oleic acid, linoleic acid 조성비는 새올콩에서만 파종기가 빠를수록 유의하게 높았다. Linolenic acid 조성비는 대부분 시험품종에서는 파종이 늦을수록 유의하게 증가하였다. 5. 파종기 이동에 따른 탄수화물과 조단백질 함량은 대부분 시험품종에서 유의차가 없었다. 그러나 새올콩은 조기에 파종할수록, 다원콩은 만기에 파종할수록 조단백질 함량이 많은 경향이었다.