The objective of this study was to evaluate efficiency of methyl-beta-cyclodextrin (MBCD) in the sperm preservation of bull. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 1, 5, 10 and 20 mM MBCD before freezing process. Analysis of viability in frozen-thawed sperm was estimated by SYBR14/PI double stain, hypoosmotic swelling test(HOST) and acrosome damage with FITC-PNA, and mitochondria activation with Rhodamin123 by flow-cytometry. The sperm viability was significantly higher in 0 mM and 5 mM concentrations of MBCD than other groups (p<0.05). However, the HOST was significantly lower at 20 mM concentration of MBCD than other concentrations (p<0.05). In addition, acrosome damage and mitochondria activation rates were significantly lower at 20 mM concentration of MBCD than other groups (p<0.05). In conclusion, the viability of sperm was not significantly different among concentrations of MBCD 0, 5 and 10 mM, but MBCD 20 mM was significantly lower than other groups. In addition, as concentrations of MBCD was high, HOST, acrosome damage and mitochondria activation rates had a negative effect in bull sperm.

난포액에 함유되어 있는 steroids와 sterol이 수정에 참여하는 정자의 주화성에 미치는 영향을 밝히기 위하여, progesterone, estradiol 17 beta 및 cholesterol이 sucrose 층으로부터 정자의 swim-up 분리에 미치는 영향을 조사하였던 바 얻어진 결과는 다음과 같다. 1. Progesterone은 정자의 이동과 운동성을 억제하였으나 수정능획득한 정자를 유인하였으며, 특히 50g/ml 수준의 progestero

This study was carried out to investigate seed germination and seedling of cabbage and sugar beet in four treatments of salinity including 0 (control), 0.5, 1.0 and 1.5％ NaCl. The results showed that different treatments of salinity had considerable effects on the germination and root and shoot length of cabbage and sugar beet. Percent of germination in both species showed significant decrease with increasing salinity up to 1.5％ NaCl. This decrease was more evident in cabbage when compared to sugar beet. The required time for germination increased with high levels of salinity. The seedling growth of both species were inhibited by all salinity levels. Particularly at 1.0 and 1.5％ NaCl, no measurable length was observed in cabbage and sugar beet. At 0.5％ NaCl root growth of both plant species was more affected as compared to shoot growth by salinity.