Aedes albopictus is an invasive mosquito that can be found in all continents. This species, considered as secondary vector of Dengue virus, has recently been suggested to play a role in the transmission of Zika virus in several countries bordering Brazil. In this study, We surveyed the distribution, abundance and main micro habitats of Ae. albopictus in Koera. The main bloodmeal source of this mosquito was mammals (71%) followed by birds (26%), and fish (1%). In flavivius detection, no virus was detected in this mosquito, but a total of six Japanese encephalitis virus (JEV) positive pools were detected from Culex orientalis and Culex pipiens. To investigate the possibility of using Wolbachia, as one of biological control strategy, we investigated the distribution of Wolbachia infection in Ae. albopicutus according to geographical distribution. Over 99% of the collected this mosquitoes harbored Wolbachia.

One of the overlooked points in mosquito blood feeding research is a final step before blood feeding. We provide the anatomical and chemosensory evidence that a piercing structure of the mouthpart of the mosquitoes is an essential apparatus for the penultimate stage in blood feeding in mosquitoes. Indeed, the stylet possesses a number of sensory hairs located at the tip of the stylet. These hairs house olfactory receptor neurons that express two conventional olfactory receptors of Aedes aegypti (AaOrs), AaOr8 and AaOr49, together with the odorant co-receptor (AaOrco). In vivo calcium imaging using transfected cell lines demonstrated that AaOr8 and AaOr49 were activated by volatile compounds present in blood. Taken together, we identified olfactory receptor neurons in the stylet involved in mosquito blood feeding behaviors, which in turn indicates that olfactory perception in the stylet is necessary and sufficient for mosquitoes to find host blood in order to rapidly acquire blood meals from a host animal.