In this study, the Euro NCAP-based AEB system evaluation simulation was conducted by applying the calculated corrected TTC by road condition estimation of scenarios (CPFA, CPNC, CPLA) of the V2P situation scenario using PC-Crash, a program used for traffic accident analysis. The scenario was evaluated in consideration of the two road conditions. the low-speed conditions among every scenarios avoided collision, but in the medium and high-speed conditions has been collided with pedestrian. It was confirmed that the time point at recognizing pedestrian was lower than set TTC at which AEB system was operated, even though the AEB was operated immediately, a collision occurred due to insufficient braking distance. As in this study, if studies such as V2V, V2P, and V2B considering road friction are actively conducted, it is expected to be useful data for automobile accident prevention and accident analysis.

Phospholipase A2 (PLA2) catalyze the committed step for eicosanoid biosynthesis and releases arachidonic acid (AA), which is oxygenated into eicosanoids that mediate immune responses in insects. Thus, any inhibition of PLA2 activity would lead to a significant immuno suppression due to lack of eicosanoids. Among more than 15 families of PLA2s, group Ⅳ cytosolic PLA2 (cPLA2) has been mainly associated with the production of eicosanoids associated with immune responses. However, no cPLA2 has been reported in all invertebrates including insects. AcPLA2 candidate gene (SecPLA2) has been identified from a hemocyte transcriptome of the beet armyworm, Spodoptera exigua. RNA interference of SecPLA2 expression significantly reduced cellular immune responses of hemocytes. When the SecPLA2 was expressed and purified, the recombinant SecPLA2 catalyzed a substrate, phosphoatidyl choline, atsn-2 position. Its catalytic activity was sensitive to pH, temperature, and calciumlevel. Furthermore, there combinant SecPLA2 was specifically sensitive to a cPLA2-specificinhibitor, methyl arachidonyl fluorophosphonate.