Phosphine (PH3) fumigation has been widely used for controlling storedgrain insect pests, causing the development of resistance of stored-grain insect pests to phosphine. PH3 resistance in Sitophilus oryzae has been reported in Korea. However, PH3 resistance in Tribolium castaneum has not been reported yet. This study was conducted to determine susceptibilities of T. castaneum collected from five different domestic locations to PH3. The susceptibility to PH3 was investigated using the FAO fumigation method. All domestic T. castaneum individuals were controlled by PH3 at 0.04 g m-3. At 0.01 g m-3, T. castaneum collected from two domestic locations did not exhibit 100% mortality. A P45S point mutation in dihydrolipoamide dehydrogenase (dld) gene was found in a PH3-resistant strain of T. castaneum (Aus07), but not in five domestic stains or a PH3-susceptible strain (Aus10). No significant difference was found in dld or cyt-b5-r gene expression across all tested strains. However, the Gyeongju-collected strain of T. castaneum showed more than a 1.7-fold increase in cyt-b5-r expression compared to the Aus07 strain. cDNA sequence analysis revealed that P45S (C133T) in the dld gene was only present in Aus07. A characteristic single nucleotide polymorphism in the cyt-b5-r gene sequence was identified in the five domestic strains. This study suggests that it is necessary to continuously monitor PH3-susceptibility of T. castaneum in Korea to quickly identify resistant individuals and prevent the spread of PH3 resistance through rapid control.

안압 측정은 농촌에 거주하는 60셰 이상의 노인올 대상으로 비접촉 안압계 (CT-80) 를 이 용하여 시 행하였다. 안압 측정 대상안은 256안(남자 : 156안， 여 자 : 100 안)으로， 그 중에서 한쪽 만 정상인 안온 29안 이었다. 대상안의 평균 안압은 15.4rnmHg로 정상 안압으로 간주되는 10 rnmHg부터 21 rnmHg 까지 법위에 포함된 대상안은 전체의 92.3% 이었고， 21 mrnHg 이상의 고안압 안온 5.8%, 10 mrnHg 이하 저안압 안은 1.9%로 고안압안이 3.9% 정도 높게 나타났다. 성 별 평균값 비교에서는 남자 15.2 rnmHg, 여자 15.6 mrnHg로 측정되어 여자가 높게 나타났고， 나이에 따른 안압의 변화는 여자가 남자보다 나이 뚫에 따라 뚜렷이 증가 하였다. 또한， 한쪽만 정상인 안온 양안이 정상인 안보다 안압(16.9 mrnHg) 이 높게 측정되었으며， 륙히 고안압 안외 비옳 (20.6%) 이 상대적으로 양안 정상인 안보다 훨씬 높온 것이 톡정책인 결과이었다. 따라서 고안압과 한쪽 눈만 정상인 안과는 많은 연 관성 있는 것으로 판단되었다.